section8_ak

section8_ak - MIT Department of Biology 7.014 Introductory...

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MIT Department of Biology 7.014 Introductory Biology, Spring 2005 Recitation Section 8 March 2-3, 2005 Molecular Biology—Transcription and Translation Today we return to take another look at the yeast cystathionine beta synthase (CBS) protein. Below is the sequence of the yeast CBS gene and surrounding DNA. We will use this sequence for parts A and B of today’s section. The sequences of both strands of the DNA duplex are shown: the top strand reads 5' to 3' left to right (1 to 2040); the bottom, complimentary, strand reads 5' to 3' right to left (2040 to 1). 5’- CAACTTCACCCAAGTAAGGATAATCAGCTCTGTCGTGACTGATAAATGC TATAT CCGGCA 1 ---------+---------+---------+---------+---------+---------+ ± 3’- GTTGAAGTGGGTTCATTCCTATTAGTCGAGACAGCACTGACTATTTACG ATATA GGCCGT ± TATGCAGTCCACACGGCATTACCGTTTCACTAATTTATTGCCATCTTCCTCCACAGTTTT ± 61 ---------+---------+---------+---------+---------+---------+ ± ATACGTCAGGTGTGCCGTAATGGCAAAGTGATTAAATAACGGTAGAAGGAGGTGTCAAAA ± GCACCGAAAGGAAAAAAAGAAACCAACACCGAAAATTTTTTTCTCCTAAAGGTTAAAGTA ± 121 ---------+---------+---------+---------+---------+---------+ ± CGTGGCTTTCCTTTTTTTCTTTGGTTGTGGCTTTTAAAAAAAGAGGATTTCCAATTTCAT ± AACGCAAGGCACTTCACCAGGCTTG TATATAT AAATGTCGTGATGCTTCTATGCCAAAGT ± 181 ---------+---------+---------+---------+---------+---------+ ± TTGCGTTCCGTGAAGTGGTCCGAAC ATATATA TTTACAGCACTACGAACATACGGTTTCA ± AAAAGGCAACACTTGAAGATTTCGTTGTAGGCCACTTGCTCAAAGGACATCTAGATAAAT ± 241 ---------+---------+---------+---------+---------+---------+ ± TTTTCCGTTGTGAACTTCTAAAGCAACATCCGGTGAACGAGTTTCCTGTAGATCTATTTA ± ACGACGTAAGAATAAAAATGACTAAAT CT GAGCAGCAA G CCGATTCAAGACATAACGTTA ± 301 ---------+---------+-------- c +-------- b +---------+--- a -----+ ± TGCTGCATTCTTATTTTTACTGATTTA GA CTCGTCGTT C GGCTAAGTTCTGTATTGCAAT ± TCGACTTAGTTGGTAACACCCC A TTGATCGCACTGAAAAAATTGCCTAAGGCTTTGGGTA ± 361 ---------+---- e ----+-- d ------+---------+---------+---------+ ± AGCTCAATCAACCATTGTGGGG T AACTAGCGTGACTTTTTTAACGGATTCCGAAACCCAT ± TCAAACCACAAATTTATGCTAAGCTGGAACTATACAATCCAGGTGGTTCCATCAAAGACA ± 421 ---------+---------+---------+---------+---------+---------+ ± AGTTTGGTGTTTAAATACGATTCGACCTTGATATGTTAGGTCCACCAAGGTAGTTTCTGT ± GAATTGCCAAGTCTATGGTGGAAGAAGCTGAAGCTTCCGGTAGAATTCATCCTTCCAGAT ± 481 ---------+---------+---------+---------+---------+---------+ ± CTTAACGGTTCAGATACCACCTTCTTCGACTTCGAAGGCCATCTTAAGTAGGAAGGTCTA ± CTACTCTGATCGAACCTACTTCTGGTAACACCGGTATCGGTCTAGCTTTAATCGGCGCCA ± 541 ---------+---------+---------+---------+---------+---------+ ± GATGAGACTAGCTTGGATGAAGACCATTGTGGCCATAGCCAGATCGAAATTAGCCGCGGT ±
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TCAAAGGTTACAGAACTATCATCACCTTGCCGGAAAAAATGTCTAACGAGAAAGTTTCTG ± 601 ---------+---------+---------+---------+---------+---------+ ± AGTTTCCAATGTCTTGATAGTAGTGGAACGGCCTTTTTTACAGATTGCTCTTTCAAAGAC ± TCCTAAAGGCTCTGGGTGCTGAAATCATCAGAACTCCAACTGCTGCTGCCTGGGATTCTC ± 661 ---------+---------+---------+---------+---------+---------+ ± AGGATTTCCGAGACCCACGACTTTAGTAGTCTTGAGGTTGACGACGACGGACCCTAAGAG ± CAGAATCACATATTGGTGTTGCTAAGAAGTTGGAAAAAGAGATTCCTGGTGCTGTTATAC ± 721 ---------+---------+---------+---------+---------+---------+ ± GTCTTAGTGTATAACCACAACGATTCTTCAACCTTTTTCTCTAAGGACCACGACAATATG ±
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section8_ak - MIT Department of Biology 7.014 Introductory...

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