Lab 3 - Lab 3 Microscopy II 1_Fluorescence_ 2.Inthelab,

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Lab 3: Microscopy II Questions for Second Microscopy Lab 1. ______ Fluorescence ___ is the property of some compounds that they absorb light at some wavelength and shortly thereafter emit light at a slightly longer wavelength. 2. In the lab, the fluorescence microscopes we use are said to be epi-fluorescence microscopes, because the illumination reaches the specimen through the ____objective______ lens. 3. _____Direct___________ immunofluorescence is the technique in which the fluorochrome is attached to the antibody directed against the molecule of interest. 4. _______Indirect_________ immunofluorescence is the technique in which the fluorochrome is attached to a "secondary" antibody directed against the "primary" antibody. 5. The ___escitation_______ filter is used to insure that only the light coming from the excited fluorochrome reaches the observer (or the camera). 6. The ____emission______ filter is used to insure that the only the light appropriate for excitation of fluorochrome reaches the specimen.
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