173f08q1 - Bio173, F08 Quiz 1 GSI _ Name _ 1. In part II of...

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Bio173, F08 Quiz 1 GSI ________________________ Name ________________________ 1. In part II of the Enzyme lab, we diluted enzyme and observed the effect on rate of reaction. Answer the questions below, assuming [ substrate ] is limiting in the reaction with full- strength enzyme, not enzyme . (note- this is the reverse of what we saw in lab) a. Draw the expected curves for absorbance vs. time and rate vs. [enzyme] under these circumstances. (two points each) b. Give an explanation for these results. (two points) 2. What function is hypothesized for polyphenoloxidase in potatoes? What prediction did this lead to in the experiment with varying levels of potato peel in extracts? (two points)
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3. Explain the following results (“Experimental error” is NOT an answer; one point each) a. After boiling the enzyme extract, no reaction is observed. b. After freezing the enzyme extract, a higher reaction rate than the never-frozen control is observed. c. The percent inhibition for an inhibitor is higher at low [substrate] than high [substrate].
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This note was uploaded on 05/08/2009 for the course CHEM 215 taught by Professor Gottfried during the Fall '08 term at University of Michigan-Dearborn.

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173f08q1 - Bio173, F08 Quiz 1 GSI _ Name _ 1. In part II of...

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