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Lecture_27_Objectives - being tested for by each “wheal...

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Lecture 27 Objectives At the completion of the lecture and the assigned reading, the student should be able to: 1) Explain the immune system mechanism responsible for each of the 4 types of hypersensitivity reactions. I – soluble Ag binds IgE on Fc(epsilon)RI = mast cell degranulation II – IgG binding to solid phase molecule that alters self, fixes C’, induces phagocytosis III – Soluble IgG immune complexes deposit on walls of small blood vessels causing C’ activation IV – Ag specific Tcells bind small peps (insect venom) or chemically modified self peps (poison ivy) 2) Explain how a “wheal and flare reaction” differs from a “delayed-type hypersensitivity (DTH)” response, and what type of hypersensitivity (I – IV) is
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Unformatted text preview: being tested for by each. “wheal and flare reaction” test type I, skin testing mark at injection and spot spreads redness, swelling DTH test type IV ex) TB test, test cellular immune response, read 48hrs 3) Define and briefly describe RIST and RAST and their usefulness in patient hypersensitivity testing. In vitro testing – for type I RIST = total IgE, traditionally a competitive immunoassay w/ radiolabeled human IgE – replaced w/ direct sandwich enzyme immunoassay RAST = allergen specific IgE, carb based solid phase assay now w/ enzymatic or fluorometric detection. Difficult b/c IgE stuck to mast cells mostly not floating in serum...
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