Unformatted text preview: being tested for by each. “wheal and flare reaction” test type I, skin testing mark at injection and spot spreads redness, swelling DTH test type IV ex) TB test, test cellular immune response, read 48hrs 3) Define and briefly describe RIST and RAST and their usefulness in patient hypersensitivity testing. In vitro testing – for type I RIST = total IgE, traditionally a competitive immunoassay w/ radiolabeled human IgE – replaced w/ direct sandwich enzyme immunoassay RAST = allergen specific IgE, carb based solid phase assay now w/ enzymatic or fluorometric detection. Difficult b/c IgE stuck to mast cells mostly not floating in serum...
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- Fall '07
- Rist, 48hrs, IgG immune complexes, allergen specific ige, Difficult b/c IgE