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Unformatted text preview: detect reactive oxygen, bacterial killing assays, chemotaxis assays, leukocyte adhesion molecule expression by flow cytometry (CD11 a,b,c and CD18) neutrophil enzyme assays Complement defect CH50 assay measures classical C path comp. EIA/ELISA detects C9 bound IgM coated microtiter wells serum C3 level 3) Explain the flow cytometry technique, including the reagent(s) that is/are used to label the individual cell population(s), and how that label is detected. Cell mixture labeled w/ fluorescent tagged monoclonal Abs specific for cell marker of interest. Cells suspended in saline ran thru cytometer, sep cells into single stream, activates the fluorochrome w/ a laser, detect fluorescent emission w/ detectors (also can simply do forward (cell size) and side (cell complexity) laser light scatter) B cell CD19, CD20 Total T cells CD3 also gamma/delta Tcells Th cells CD3+CD4 Tc cells CD3+CD8 NK cells CD16 OR CD56...
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- Fall '07