Exam2_key_04

Exam2_key_04 - BISC 320 Midterm Exam 2 -- 10(22104 Total...

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Unformatted text preview: BISC 320 Midterm Exam 2 -- 10(22104 Total Score (out of 100 pts) Multiple Choice (3 pts. Each) Choose the BEST answer! 1) Why is cohesion necessary for proper chromosome segregation during mitosis? a for genetic exchange (crossing over) ® to ensure the two sister chromatids are segregated into different daughter cells c) to ensure sister chromatids are segregated together into the same daughter cell d) to make sure homologous chromosomes segregate into different daughter cells 2) DNA sequencing requires: a) DNA polymerase b) Dideoxynucleotides c) Deoxynucleotides Q) All of the above 3) In a haploid cell, which of the following does not occur: a) mitosis b) homologous recombination @ meiosis ) cohesion 4) When comparing the genomes of different organisms, gene density is found to: a) increase with increasing complexity of the organism (h) decrease with increasing complexity of the organism c) is essentially constant among all organisms d) it varies but there is no clear relationship to the complexity of the organism 5) Which of the following is 'not involved in processing of Okazaki fragments: 21) DNA polymerization b) DNA ligation c) Endonuclease or exonuclease @ RNA polymerization 6) In prokaryotic cells, detection of hemi—methylated DNA is required by which of the following processes: a) homologous recombination @> mismatch repair c) photoreactivation of thymine dimmers (:1) base excision repair 7") Making a specific gene deletion in any organism requires: a) transposon mutagenesis b) knowledge of the gene’s function c a haploid cell -' cl homologous recombination (3;) 8) A diploid yeast cell has a normal and a mutant allele of a gene involved in biosynthesis of histidine. A haploid yeast cell with the mutant allele of this gene is unable to grow without histidine added to its growth medium; however. the diploid cell described above is m to grow without added histidine. The ability of the diploid cell to grow without add histidine is due to: @ Complementatiou ) DNA repair c) Homologous recombination d) All of the above 9) (6 pts.) During DNA replication, DNA polymerase incorporates a base incorrectly into the nascent DNA. Name the main mechanism that will act to correct the error. Then assuming the first mechanism fails, name the second mechanism that will act to correct the error. Be sure to indicate which is first and secoud. Q 1) Praewdi'r‘g (3’ iv 9’ €¥0nctc(?au) @213 Misflwbh papa? 10) ( pts.) Name or briefly describe: ,,--_ a) the enzymatic activity of telomerase that allows it to extend the telomeres {-2} Fer/W56 itfifiS’C r 17f? s? i. - b) which DNA strand is extended by telomerase‘? £21) 3! W l 'lfiilw":-"="“W E'fi‘ 3" .' f c) the nature of the telomeric DNA sequence that is necessary for tclomerase to act 5-3? 3+ 15 (‘2 peas re 5'. d) the physical component of telomerase that allows it to bind telomeres specifically A‘ . I 11) (12 pts.) From each of the following questions, choose the BEST answer from the follOwing list of enzymatic activities. Base your answer on the enzymatic activity at the DNNRNA level (i.e. the specific chemical reaction that is carried out) List to choose from: DNA glycosylase, DNA polymerase, RNA polymerase. DNA ligase, Type I DNA topois'opmerase, Type 11 DNA T0poisomerase, DNA recombinase, DNA endonuclease, 5’ to 3’ DNA exonuclease, 3’ to 5’ DNA exonuclease, DNA helicase, DNA Primase 6) t a) Which activity is the opposite ofDNA polymerase? 3' +0 5" emetic?“ £3???le b) Which activity is the opposite of DNA endonuclease? 9“ fi- [35 t 54 c) Which shares similar activities as Type II DNA Topoisomerase? b (\l A (eco mbmé '5»? ~i’ ?i;..$<""’l=5;.“:.;'j,ff (1) Which is similar to DNA Primase? w Pr QAVFM S;- (Ll) f 12) (12 pts.) Draw the structure of a DNA replication fork showing template and nascent strands. Use lines for each DNA strand and indicate their orientations (5‘ or 3‘). Also use anowheads on the ends of nascent strand to indicate their direction of synthesis. Indicate the leading and lagging strands and show at least two Okazaki fragments on the lagging strand. Indicate which Okazaki fragment was synthesized first, and which was second? how RNA primers. Do not try to show proteins. 13} (10 pts.) a) Describe the basic structure of a nucleosome in detail (name proteins, how many. how much DNA). /’L I 1‘, (4? b0 0? 91m" are uraka {fig “FMS are..sz as (am hi3?” Wei. PfiFchJoOlnTCb cm‘mr 2 me 04 new, H214,H26; Z off“; 5 *Gdhfifiw H5, 51"!" H3~H¥ b) Describe two additional hierarchies of chromatin structure. In each case, name andfor briefl describe the basic structurez’or anization of the DNAJ'chromatin and name an proteins or protein structures involved. 30 Am 'P'idrrh'i' is a from (we!!wa 5mm Wm nuciéosamai _______—-—-—-_——'—- mAgAnucieojah-t DNA Him i” bf‘flJinn m link/V ('6ij £9.3th (106(605W5 Wakinj é th'i'fifj of hertch (whdw'hmo Char-«hm imvpf or Amafifg (31ij (“gig/Info? DNA W6 binned 3L” 5 fit 5MPfi9i¢fl rl—y «(arm (we; of ohfq-rflfl'hn ___,______. arc-what .4 mach“ £205» cheMEn w'fi rrvj—ghpeeaflfaifiin “but 5%; Oéfsmané'n Wm” “’me W W“ $521112: Last Name (print) First Name {print} 14) (10 pts.) Draw the structure of a Holliday Junction between the following two homologous DNA molecules. Make sure you indicate 5’ and 3’. The arrows indicate the site of single-strand nicks that initiate the Holliday Junction. Make a second drawing after migration of the Holliday Junction to give a heteroduplex at region B. Finally, use mom on your second drawing to indicate the position of cleavage to resolve the Holliday junction without a crossover. 5, A B o 3 1‘ B D 3. a Jr b d 5’ a b d 9., A 5 3. 15) (12 pts.) List the basic reagentsfmolecules needed to carry out a PCR reaction, and briefly describe the three basic steps in the PCR reaction itself W perms: CW) 9Wn'fific'hma w §£VarénLr$Vlwfi 4M4}; of” Wén‘r ®NA (Unfit W m fist a: Aflngalumf '- {Dwa- W NSZJ'C 629 film ecfmm‘ the finned 9 +0 swath 16) (6 pts.) Show the structure of [he molecule(s) that result from a single crossover between the homologous regions, indicated by arrows. Indicate the genetic markers (A, B, C, and D) on the resulting molecu1e(s). A W—‘B C—-> D ...
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Exam2_key_04 - BISC 320 Midterm Exam 2 -- 10(22104 Total...

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