L12 - Metabolism Glycolysis Topics Glycolysis and Krebs...

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Unformatted text preview: Metabolism Glycolysis Topics Glycolysis and Krebs Cycle Energy Transformation Compounds Pathways (catabolic, anabolic & amphibolic) Substrate Level Phosphorylation Glycolysis Preparing for Fermentation or Oxidation 1 The Important (Exam) Stuff Describe energy transformation compounds involved in glycolysis. Explain the differences between anabolic, catabolic and amphibolic pathways. Describe microbial transport in glycolysis. Describe substratelevel phosphorylation. Describe the products of glycolysis in terms of carbon compounds, ATP (net and total) and NADH. Explain the Disease O' the Day The MB 351 Simplified Version Glycolysis Krebs Cycle Fermentation ETC NADH ATP Carbon Flow 2 Energy Transformation Compounds What are Pathways? Sequences of reactions catalyzed by enzymes Each step is usually catalyzed by a separate enzyme Some pathways are catabolic Some pathways are anabolic Some pathways are amphibolic The complete version 3 What is Glycolysis? Anaerobic & Ancient Means "Sweet lysis" It occurs in most living cells 1st stage in sugar metabolism Discovered by EMP (EmbdenMeyerhofParnas) Involves 10 enzymes, 10 steps Occurs with or without O 2 Substratelevel phosphorylation ATP generated + NADH+H generated First Step: Get Sugar! 4 Preparatory 6Carbon Stage ATP PEP ADP P Glucose bacterial PTS Glucose 6phosphate (G6P) Fructose 6phosphate (F6P) Fructose 1,6diphosphate (F16DP) isomerase ATP ADP P P kinase P aldolase P P Dihydroxyacetone phosphate Glyceraldehyde 3phosphate DHAP G3P ATP Generating 3Carbon Stage NAD+ + Pi NADH ADP ATP P P P glyceraldehyde 3phosphate (G3P) dehydrogenase P 1,3diphosphoglyceric acid (1,3DPGA) kinase P 3phosphoglyceric acid (3PGA) 2phosphoglyceric acid (2PGA) H O 2 ADP ATP P enolase phosphoenolpyruvic acid (PEP) kinase pyruvic acid (pyruvate) x2 Used to create fermentation products 5 The only way to generate ATP in the absence of oxygen. A phosphate group is transferred to a pathway intermediate and eventually transferred to ADP creating a Highenergy phosphoanhydride bond. Glycolysis From a Distance INVEST HARVEST 6 Amphibolic Pathways F E D Catabolic only Anabolic only G Means "to change both ways" Catabolic and anabolic pathways are not always identical Some enzymes are shared while some are unique (regulation) Biosynthetic reactions need to be largely irreversible and are often coupled to ATP hydrolysis C B A Glycolysis and Gluconeogenesis Glucose A ADP H O 2 A ADP H O 2 Glycolysis ADP Gluconeogenesis A PEP ADP CO 2 CO 2 GDP G ADP A A Pyruvate 7 What happens next? Glycolysis Krebs Cycle Fermentation ETC NADH ATP Carbon Flow When Oxygen is present Glycolysis Krebs Cycle Fermentation ETC NADH ATP Carbon Flow 8 Text References th Pages 117123, 126128 (11 Edition) Pages 122128 (10th Edition) 9 ...
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This homework help was uploaded on 03/21/2008 for the course PY 211 / 212 taught by Professor Chilton during the Spring '08 term at N.C. State.

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