BIOL2070H_Lab_4_2018_fillable(1).docx - Lab 3 and 4...

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Lab 3 and 4 AssignmentThis value of this assignment is 35 marks and it is worth 10% of your final grade.Table 1. Volumes that will be used to generate solutions containing a known concentration of BSA (7 marks)LabelBSA concentration(µg/ml)Volume ofstock BSA(µl)Volume of0.15M NaCl (µl)Total Volume (µl)A595 nmTube Set 1A595 nmTube Set 2A595 nmAverageTube 100100100000Tube 225100Tube 350100Tube 475100Tube 5100100Calculations (2 marks)Table 2. Using a standard curve to determine the protein concentration of an unknown sample (6 marks)
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Table 3. Volumes that will be used to generate solutions containing a desired protein concentration

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