lecture 12 - BIO 311C Not all compartments in a living cell...

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BIO 311C February 13, 2008
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Not all compartments in a living cell are at the same pH value. Textbook Fig. 6.9, p. 100 The cytoplasmic matrix and most compartments are near neutral pH ( 7.4). Lysosomes are at an acidic pH (2). The Mitochondrial matrix is at a alkaline pH (8). *
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acidic compartment of a cell such as a lysosome neutral or basic compartment of a cell such as the cytoplasmic matrix Effect of pH of a Cellular Compartment on the Ionization State of a Carboxyl Functional Group * R O O C H R O O - C
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acidic or neutral compartment of a cell such as the cytoplasmic matrix basic compartment of a cell such at the mitochondrial matrix Effect of pH of a Cellular Compartment on the Ionization State of an Amine Functional Group * R H H N H + R H N H
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* Effect of pH of a Cellular Compartment on the Ionization State of a phosphate Functional Group neutral compartment of a cell acidic compartment of a cell basic compartment of a cell
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Many Biological molecules have positive and negative charges simultaneously. Consider a molecule in the cytoplasmic matrix of a cell ( ~pH 7) that contains three ionizable functional groups : Note: This molecule would have 3 electric charges about half of the time and 4 electric charges about half of the time. organic molecule *
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If only one or a very few molecules with an ionizable functional group are placed into a compartment of a cell, then the pH (the relative amounts of H 3 O + and OH - ) of the compartment will determine the ionization state of the functional group. If a large number of molecules with an ionizable functional group are placed into a compartment of a cell (a large enough number of molecules to make its concentration as high or higher than the concentration of
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lecture 12 - BIO 311C Not all compartments in a living cell...

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