lecture 19 - BIO 311C February 29, 2008 Exam 2 will be held...

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BIO 311C February 29, 2008 Exam 2 will be held in this room during the class period on Friday 7 March. Exam 2 will cover the topics listed on the Lecture & Reading Schedule from Feb. 6 topics through the Mar. 3 topic (“Introduction to biological molecules” through “Transport across biological membranes”).
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+ H 2 O Formation of a Dinucleotide from Two Nucleotides * 5‘ end of nucleotide 3' 5' 5' 3' 3' 5' 3‘ end of nucleotide 3‘ end of dinucleotide 5‘ end of dinucleotide 5' 3' 3' Review
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This trinucleotide 5’ end 3’ end * A C G 5’–A–C–G–3’ Can be written as: or: 5’–ACG–3’
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Examples of a primary Structure of Polynucleotide Chains (shown in abbreviated forms) C A U C G A 5' 3' * dT dC dT dA dG dG 5' 3' A polyribo nucleotide chain used for constructing RNA A polydeoxyribo nucleotide chain used for constructing DNA This sequence may be shown as: C A A G C U 5' 3' dT dG dC dG dA dT 5' 3' This sequence may be shown as: Note: sometimes the “d” is not shown in front of the nucleoside sequence if it is understood that the molecule is a polydeoxyribonucleotide.
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5' 3' 3' 5' end 3' end 5' Directionality of a Polynucleotide Chain The "backbone" of a polynucleotide chain consists of alternating sugar and phosphate. The nitrogen bases project from the polynucleotide chain much like R-groups project from a polypeptide chain. Can you distinguish the pyrimidine bases from the purine bases in this illustration? phosphate sugar * From textbook Fig. 5.25, p. 87 Abbreviated Structure of a Polynucleotide Chain 3' 5' direction 4’ 2’ 1’
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Consider a polynucleotide with a nucleotide sequence of: 5' 3' A U C G A G C U 5' 3' * Would this molecule be a component of DNA or would it be a component of RNA? Does this molecule contain ribose or does it contain 2-deoxyribose?
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Consider a second polynucleotide chain with an internal nucleotide sequence of: 5' 3' A U C G A G C U 5' 3' 3' 5' A G C U
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lecture 19 - BIO 311C February 29, 2008 Exam 2 will be held...

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