What is the need of adding DNS solution to the enzyme...

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1. What is the need of adding DNS solution to the enzyme substrate solution?
2. What is the problem in determining rates at low substrate concentration?
3. Draw the Michaelis Menton plot and the Line Weaver Burk Plot using the data given below and find the Vmax and Km from the Plot.
where, y = 1/ V 0 , x = 1/ [S], Intercept, c = 1/ V max , Slope, m = K m / V max Trendline (linear regression) equation for “No- inhibitor” from LB plot y = 35.63x + 0.3861 Now, from Intercept, c = 1/ V max Or, 0.3861 = 1/ V max Or, V max = 1/ 0.3861 = 2.59 Hence, V max = 2.59 umol min -1 mL -1 Now, Km = m x Vmax = 35.63 x 2.59 =

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