Mock IA II Hydrogen Peroxide Rate of Reaction.docx

Mock IA II Hydrogen Peroxide Rate of Reaction.docx - The...

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The effect of substrate concentration on the rate of reaction of enzyme activity MOCK IA II Nuno Gomes Biology / Grade 11 / Mr. Barrick / IA
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Table of Contents: Background Information: ......................................................................................................... 3 Research Question: .................................................................................................................. 3 Hypothesis: ............................................................................................................................. 3 Variables: ................................................................................................................................ 4 Independent Variable: ........................................................................................................................ 4 Dependent Variable: .......................................................................................................................... 4 Controlled Variable: ........................................................................................................................... 4 Health/Safety/Technical Notes: ............................................................................................... 5 Apparatus: ............................................................................................................................... 5 Diagram: .................................................................................................................................. 6 Method: .................................................................................................................................. 6 Raw Data (Quantitative): ......................................................................................................... 7 Processed Data: ....................................................................................................................... 8 Table of Figures: Figure 1- The reaction trend when an enzyme is present ............................................................... 3 Figure 2- Rate of Reaction and Hydrogen Peroxide Concentration ................................................. 4 Figure 3- The General Diagram for the Experiment ......................................................................... 6 Figure 4- Graph showing amount of oxygen released ..................................................................... 8 Figure 5- Graph resembling the rate of reaction ............................................................................ 9 Figure 6- Graph showing the Standard Deviation without outliers ............................................... 10 Table of Tables: Table 1- Table of Amount of Oxygen Released ................................................................................ 7 Table 2- Table including the rate of reaction of the oxygen produced ............................................ 8 Table 3- Shows table with Standard Deviation of the results provided ........................................... 9
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Background Information: Enzymes are by definition biological catalysts that are able of catalyzing different biochemical reactions in a living cell. During catalyst reactions, binding between the substrate and the enzyme occur in the active site, so that an end product can be obtained. [GBi16] Catalase is an enzyme produced by our liver to break down Hydrogen Peroxide (substrate) into the following: H 2 O 2 →O 2 + 2 H 2 O Since this is a decomposition reaction, it is exothermic. Although the hydrogen peroxide has the ability of degenerating itself, the use of catalase allows this reaction to occur at a much faster rate, also decreasing the activation energy (the minimum energy barrier that hydrogen peroxide molecules have to overcome its decomposition). [Far] The following diagram can be used to show the reaction trend when enzyme is present: Figure 1- The reaction trend when an enzyme is present Since the enzyme lowers the activation energy, the rate of reaction increases without consuming enzyme. A gas syringe will be used since the reaction produces oxygen gas (as shown in the previous equation). Naturally, the faster the reaction, the faster the increase of pressure. Therefore, by calculating the total amount of oxygen gas produced due to the reaction, the rate of reaction will be calculated and furthermore analyzed. Research Question: To investigate the effect of Hydrogen Peroxide (manipulating the substrates concentrations) on the rate of yeast activity (enzyme activity of catalase), on the decomposition of hydrogen peroxide.
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Hypothesis: If the quantity of enzyme and substrate is kept constant, differing substrate concentrations will determine the rate of reaction. The higher the concentration of hydrogen peroxide, the more catalase will be catalyzed in the time given, and therefore more oxygen will be produced.
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