4126.pdf - Description ProductSheet (ATCC4126...

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Strain Designation: [BI CZAS 321/7, CBS 1200, CCRC 21494, CCY 21­4­26, VTT C­79092] Deposited Name: Saccharomyces anamensis Will et Heinrich Product Description: An ampoule containing viable cells (may include spores and mycelia) suspended in cryoprotectant. The information recommended in this section is to assist users in obtaining living culture(s) for their studies. The recommendation does not imply that the conditions or procedures provided below are optimum. Experienced researchers may initiate the growth of a culture in their own way. ATCC ® Medium 200: YM agar or YM broth ATCC ® Medium 1245: YEPD Growth Conditions Temperature: 30°C Atmosphere: Typical aerobic Recommended Procedure For freeze­dry (lyophilized) ampoules: 1. Open an ampoule according to enclosed instructions. 2. From a single test tube of sterile distilled water (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a sterile pipette and apply directly to the pellet. Stir to form a suspension. 3. Aseptically transfer the suspension back into the test tube of sterile distilled water. 4. Let the test tube sit at room temperature (25°C) undisturbed for at least 2 hours ; longer (e.g., overnight) rehydration might increase viability of some fungi. 5. Mix the suspension well. Use several drops (or make dilutions if desired) to inoculate recommended solid or liquid medium. Include a control that receives no inoculum. 6. Incubate the inoculum at the propagation conditions recommended. 7. Inspect for growth of the inoculum/strain regularly. The sign of viability is noticeable typically after 1­2 days of incubation. However, the time necessary for significant growth will vary from strain to strain.
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  • Winter '15
  • ATCC, Ribosomal RNA gene

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