CC LAB ELEC.docx - 1 | J E Sese 3CMT Buffer Reagent Calcium...

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Calcium Magnesium Chloride Phosphorus Buffer Lysine buffer (pH 11.1) Sodium azide Stand for 10 mins read within 60 mins. Stand for 5 mins in the dark read within 60 mins. Stand for 1 min at room temp read within 60 mins. Reagent 8 – hydroxyquinolone O – Cresolphthalein complexone HCl *read after 5-50 mins. TRIS buffer (pH 11.0) Potassium carbonate GEDTA Xylidyl blue Sodium azide Activators 2,4,5 – Tri – (2 - pyridyl) – s – triazine (TPTZ) (partially as mercury (II) complex) Iron (II) sulphate * RGT IS PHOTOSENSITIVE! DO NOT EXPOSE TO LIGHT Ammoniumheptamolybdate Sulphuric acid (pH <1.0) Detergent Activators and stabilizers Standard Calcium: 8 mg/dL or 2 mmol/L Sodium azide Magnesium: 2.5 mg/dL or 1.03 mmol/L Sodium azide Chloride: 100 mmol/L or 355 mg/Dl Phosphorus: 10 mg/dL or 3.2 mmol/L Principle Ca ions + O – Cresolphthalein complexone in alk medium = purple complex = Ca conc Alk medium: Mg + Xylidyl blue = colored complex. Inc in absorbance prop to Mg conc. Cl + TPTZ = mercury (II) chloride; liberated TPTZ + Ferrous ions = blue complex Absorbance change = Cl- Strong acidic medium: Phosphate + Molybdate = complex -> abs in the near UV = phosphate conc Wavelength 570 nm 520 nm 590 nm 340 nm Optical Path 1 cm 1 cm 1 cm 1 cm Temperature 20 - 25°C 20 - 25°C 20 - 25°C 20 - 25°C Measuremen t Against reagent blank Against reagent blank
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  • Fall '14
  • Sodium, Ion, mins, J. E. Sese

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