What Exactly is in Your Food Lab__Youssef.docx

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Running Head: What Exactly is in Your Food? Youssef 1 What Exactly is in Your Food? Youssef Youssef Elearning Thames Valley District School SBI 4UV-02 Ms. Bracewell February 16, 2018
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Running Head: What Exactly is in Your Food? Youssef 2 What Exactly is in Your Food Laboratory? Purpose: The purpose of this laboratory is to investigate if nutrients such as carbohydrates like monosaccharaides and polysaccharides, protein, and lipids are present in six different unknown food samples by using four different indicators: Benedict’s solution, Lugol’s Iodine solution, Biuret’s solution, and Sudan IV Red solution. Hypothesis: If all the different unknown foods are different samples then they will all test different for various nutrients (monosaccharaides, polysaccharides, protein, and lipids) with some testing positive for multiple nutrients because some foods contain multiple nutrients together. Materials: Dropper Rubber Gloves Biuret’s solution Test Tube Rack Safety Goggles Lugol’s Iodine Solution Food Samples (A-F) Notepad Sudan IV Red Six Test Tubes Labeled A-F Benedict’s solution 500 mL beaker and hotplate (hot water bath) Glass Stirring Rod Safety Lab Coat Timer Procedure: Part A: Setting Up 1. Make and record physical observations for each indicator on an observations table (notepad) (Indicators include: Benedict’s solution, Biuret’s solution, Lugol’s Iodine solution, and Sudan IV Red solution). 2. Determine the conditions or colors of the reaction for a positive test of a certain nutrient for each chemical indicator and note on an observations table.
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Running Head: What Exactly is in Your Food? Youssef 3 3. Follow all safety procedure such as wearing a pair of safety goggles to avoid chemicals making contact with eyes, wearing a lab coat to prevent any substances from staining clothes or making contact with skin and use gloves to avoid contact with the hands 4. Label 6 test tubes with a letters A through F 5. Fill up the six test tubes with the unknown food samples provided accordingly , and place them on the test tube rack in alphabetical order 6. Fill up a 500-mL beaker with water and place it on a hot plate to set up the hot water bath. Part B: Simple Carbohydrate (Monosaccharaides) Test 1. Using a dropper, add a couple drops of Benedict’s solution to each test tube containing an unknown food sample. 2. . Heat up each of the test tubes individually in a hot water bath for three minutes. Use the stop watch to time this. 3. After heating, place the test tubes on the test tube rack and record the change in colour of each sample on an observation table. 4. Clean each test tube properly to prevent contamination and then put the new food samples into each corresponding test tube.
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Running Head: What Exactly is In Your Food? Youssef 4 Part C: Complex Carbohydrate (Polysaccharides) Test 1. Using a dropper, add about two drops of Lugol’s Iodine solution to each test tube containing the unknown food samples.
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  • Winter '12
  • Almanke
  • Biology, Nutrition, Carbohydrate, Test Tube, food samples, Youssef Youssef

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