gel electrophoresis.rtf - The Principles and Practice of...

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The Principles and Practice of Agarose Gel Electrophoresis Aviva Lerman February 6, 2018 Block: D Ms. Bloch Biology 1A Sam and Max “On my honor, I have neither given nor received any unauthorized aid on this lab report.”
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Data: Number of Components (Bars), Distance Travelled, Charge of Each Dye, and Color Change Sample (Color) Number of Bands Distance of Each Band from Gel Charge Color Change? Sample A (orange) 1 2cm Negative (-) No Sample B (purple) 1 1.2cm Negative (-) No Sample C (red) 1 1.5cm Negative (-) No Sample D (blue)
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1 0.6cm Negative (-) No Sample E (mixed) 3 Band 1: 0.5cm Band 2: 1cm Band 3: 1.5cm All negative (-) Yes- separated into 3 colors (blue, purple, and orange, respectively) Sample F (purple/blue) 2 Band 1: 0.5cm Band 2: 1cm Band 1: negative (-) Band 2: positive (+) Yes- separated into 2 bands but they were both blue Analysis: It appears as though agarose gel electrophoresis separates molecules based on how many components it has. For example, samples A, B, C, and D each only has one band because
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