Lab Report 1.docx - Cailin Winokur June 11,2018...

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Chapter 4 / Exercise 10
Biology
Martin/Solomon
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Cailin Winokur June 11,2018 Microbiology Lab 1: Microscopy for Microbiology- Use and Function Introduction: Throughout the experiment, there will be a total of three different exercises. It will being with exercise one with getting to know your compound Microscope/ In this part of the lab, you will review all parts of the microscope and calculate Field of View. Then you will use the E slide to practice focusing. You will need to switch your 4x objective for the 100x objective at the end. Next will be exercise two with viewing Prepared Microbe Slides n this part of the lab you are asked to view the following slides: Amoeba proteus , Yeast, Penicillium, Bacteria Bacillus form, Bacteria Coccus form, and Bacteria spirillum at both 4x, 10x, 40x and 100x. Take photomicrographs (looking through the ocular lens of the microscope) using best objective for viewing. The photos must be included in the lab report. Use the photos included in Exercise 2 to help you identify the organisms. Finally, exercise 3: Wet-Mount Slides You will prepare a wet mount slide of cheek cells and tooth plaque and observe them under the microscope. You will prepare a wet mount slide of cheek cells and tooth plaque and observe them under the microscope. Exercise 1: Getting to Know your Compound Microscope
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Chapter 4 / Exercise 10
Biology
Martin/Solomon
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Data Table 2: Total Magnification Lens Ocular Magnification Objective Magnification Total Magnification Scanning 15x 4x 60x Low Power 15x 10x 150x High Power 15x 40x 600x Oil Immersion 15x 100x 1,500x Data Table 3: Field Of View Letter Component Component Function A Ocular Lens Transmits and magnifies the image from the objective lens to your eye. B Main Tube Curved portion of microscope frame that holds all of the optical parts at a set distance from the stage C Turret / Nosepiece A rotating amount at the bottom of the tube that holds three objective lenses D Objective Lens Gathers and focuses light from the specimen. E Condenser Lens Lens system under stage that aligns and focuses the light from the lamp onto the specimen. F Stage Platform where the specimen rests G Diaphragm Under the stage, the diaphragm has holes in it to let varying degrees of light in under the specimen.

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