0009.docx - 20 The is widely used method to separate...

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20. The _____________ is widely used method to separate individual bacteria from mixtures. Isolation Streak Plate 21. (T/F) The goal of isolation streak plates is to get individual bacterial colonies from a mixture.True 22. Which one of the following is the correct method for streaking a plate? Sterilize the wire loop. Cool the loop by touching it on the edge of the sterile agar plate. Dip the loop into the broth culture containing the mixture of bacteria. Lift the lid of the plate just enough to insert the loop. Drag the loop over the surface of the top one-third of the plate back and forth in a "zig-zag" formation. The loop has picked up thousands of bacteria which are spread out over the surface of the agar. Sterilize the loop in the flame. Turn the plate 90 degrees and drag the loop through the area you have just streaked two to three times and continue to drag the loop in a "zig-zag" formation in the remaining half of the plate without touching that area again. Sterilize the wire loop.
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  • Spring '10
  • RIEBLING
  • Growth medium, loop, Agar plate, streak, Streaking

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