Unit 4 activities .docx - 1 Given the following mutations...

1. Given the following mutations, predict whether the mutant would be Lac
+
or Lac
-
. (5
points)
a. Point mutation in lacZ
b. Deletion of the operator sequence
1. -
c. A transposon inserted into lacI
1.
d. A frameshift mutation in lacY
e. A deletion of lacA
1.
2. Given the following mutations, predict whether the mutant would be Trp
+
or Trp
-
. (6
points)
a. A frameshift in trpR
b. A deletion of the loop 2 region in the leader peptide
c. A deletion of the two trp codons in the leader peptide. Assume that the leader
peptide RNA behaves normally otherwise.
1. -
d. A point mutation in trpE
1. -
e. A deletion of the loop 3 region in the leader peptide
1.
f. A mutation such that the allosteric site of anthranilate synthase behaves as if
tryptophan is always bound
1. -
3. Given the following mutations, predict whether the mutant would be able to sporulate or
not. (5 points)
a. A deletion of spo0F