LH 1 - Essentials of Molecular Biology Chapters 7 and 10.1...

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Essentials of Molecular Biology Chapters 7 and 10.1 – 10.6 (pages 166-204, 257-268) Laura K. Harris, M.S. oesterei@msu.edu
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Central Dogma of Molecular Biology http://pni.psychiatry.ohio-state.edu/jkg/pdf/ 52.pdf
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Central Dogma of Molecular Biology Secreted from cell Inserted in cell wall Perform cell functions Perform replication
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Outline Lesson 1: Review of DNA structure (pgs 167-171) DNA supercoiling (pgs 171-176) DNA replication (pgs 176-181) Lesson 2: Transcription (pgs 188-193) Translation (pgs 193-204) Lesson 3 (pgs 182-188) : Mutations (pgs 257-266) Recombination (pgs 267-268)
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Differences between DNA and RNA? D N A m R N A
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What is the Structure of DNA? Antiparallel – one strand runs 5’ to 3’, the other strand runs 3’ to 5’ Complementary – nucleic acid sequences that can base-pair with each other
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What is the Structure of DNA? Proteins interact with DNA mainly by binding to atoms of bases exposed in the major groove
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How are Genes Arranged? Open Reading Frames 1 2 3 1 2 3 Open reading frame – DNA sequence that could be translated to yield a protein of known length and composition Polycistronic mRNA – a single mRNA encoding for more than one protein Operon – cluster of genes which transcribe into a polycistronic mRNA P O
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How are Genes Arranged? Splicing and Alternative Splicing
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This note was uploaded on 04/15/2008 for the course MMG 301 taught by Professor Mulrooney during the Spring '06 term at Michigan State University.

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LH 1 - Essentials of Molecular Biology Chapters 7 and 10.1...

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