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Unformatted text preview: -100ul for 4uM final concentration-50ul for 2uM final concentration Hanks Salt Solution: per 1L-Hanks w/o Ca or Mg, Sigma H2387-350 mg NaHCO3-.238g -1mM Hepes to stabilize PH-pH to 7.3-7.4 w/ NaOH Hanks w/20% FBS : for 500ml add 100ml of FBS aliquots Dissociation Solution :-Hanks Salt Solution + 12 mM-12mM MgSO4 (7H2O) (.295g/100ml) Digestion Solution:-137mM NaCl .8g/100ml-5mM KCL .037g/100ml-7mM Na2HPO4 .099g/100ml-25 mM HEPES .6g/100 pH 7.2 w/NaOH Matrigel : Dilute 1:50 in sterile medium of choice, store @4C....
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This note was uploaded on 03/26/2008 for the course CHEM 237 taught by Professor Yu-chinli during the Fall '07 term at Texas A&M.
- Fall '07