2004_Midterm - Chemistry 3510-090 (Online Biochemistry)...

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Unformatted text preview: Chemistry 3510-090 (Online Biochemistry) Midterm Exam (2 hours) Summer 2004 Part 1 (20 pts) _______ Part 2 (48 pts) _______ Part 3 (11 pts) _______ Part 4 (21 pts) _______ Total (100 pts) _______ Potentially Useful Information pH = p K a + log([A- ]/[HA]) 1 Torr = 1 mm Hg G = H T S For A + B C + D G = G + RT(log K eq ) R = 1.987 x 10-3 kcal mol-1 K-1 K eq = ([C][D]/[A][B]) k 1 k 3 For E + S ES E + P k 2 V = k 3 [ES] V max = k 3 [E T ] K M = ( k 2 + k 3 )/ k 1 V = V max ([S]/([S] + K M )) 1/V = 1/V max + ( K M /V max )(1/[S]) log 0.1 = -1 log 1 = 0 log 10 = 1 log 100 = 2 Part 1. Multiple choice with a twist. For the following 10 questions, circle ALL of the correct answers. Note that many of the questions do, in fact, have multiple correct answers. (2 points each) 1) Which structure(s) is/are found in a strand of DNA? a) Guanine b) Cysteine c) Adenine d) Uracil e) Deoxyribose f) Pearls 2) An important thing to understand in Biochemistry is the relative size of things. What is the approximate length of a peptide bond? a) 1 to 5 b) 5 to 10 c) 10 to 50 d) 0.1 to 0.5 nm e) 50 to 100 pm f) 1 to 5 parsecs 3) Proteins can perform a variety of important functions. Which of the following are functions that can be attributed to proteins? a) Transfer of chemical groups (such as phosphoryl) between different biological compounds. b) Shifting the equilibrium of a chemical reaction to favor the products. c) Synthesis of proteins. d) Breakdown of proteins. e) Everything that is claimed on the 2:00 AM infomercial. 4) Which of the following are important aspects in the design of PCR primers? a) The primer must anneal to only one portion of the template DNA. b) The primer must not contain any long runs (greater than 10) of a single nucleotide. c) A single primer must contain bases that are complimentary with bases in both ends of the template DNA so that the reaction will proceed in both directions. d) The primer must contain at least one of all four of the DNA nucleic acids. e) The primer must contain a set of nucleic acids that yield a proper annealing temperature. f) The primer must wear its best gown to the prom on Saturday night. 5) Which of the following could allow a scientist to study the spatial atomic structure of a protein? a) Treatment with CNBr. b) NMR c) Mass spectroscopy d) X-ray crystallography. e) Size Exclusion Chromatography f) A very large magnifying glass. 6) DNA ________________. a) consists of ribose, a phosphate backbone, and a base (guanine, cytosine, thymine, or adenine) b) in the double stranded form always has a C:T ratio and A:G ratio of 1 c) is susceptible to strand cleavage by a class of enzymes called restriction enzymes....
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2004_Midterm - Chemistry 3510-090 (Online Biochemistry)...

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