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Lab Report 4B Streak Plate Method.docx - BIO 310, Lab...

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BIO 310, Lab SectionProfessor Gonzalez-PedrosaMarch 29, 2018Title of Report: An Introductory Exposure to Streak Plate Method (Lab Report 4B)Introduction/ContentThis experiment utilizes a mixed culture, which contains two different bacteria species. A mixed culture isused in hopes of isolating one from the other and eventually isolated a pure culture. The bacteria specieswere identified in this mixed culture; however, this technique is used in laboratory and medical settingsin order to identify unknown pathogens or microorganisms. The mixed culture is applied to an agar plate,which provides the bacteria with a nutrient supply and a means to grow and divide. The streakingmethod is a successful process for isolating an individual species because the cell density decreaseswithin each quadrant and cells have more space to grow into isolated colonies.It is useful to understand that colonies of bacteria may grow from an original cell, a pair of cells, a chainof cells, or a cluster of cells. Since the origin is not able to be detected, we call the origin cell type a“colony forming unit,” or CFU.Streak plating can also be completed in a variety of techniques. The patterns most commonly used are(1) the quadrant method (the plate is separated into four parts); (2) T-streak (the plate is separated into aT-shaded portion); and (3) zigzag (the plate is covered in a continuous streak within the entirety of theplate).

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Term
Spring
Professor
NoProfessor
Tags
Microbiology, Bacteria, Agar plate, streak plate, inoculating loop, streak, Streaking

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