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Genetics-Review for Exam 2

Genetics-Review for Exam 2 - Genetics Fall 2007 Things to...

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Genetics – Fall 2007 Things to Know for Exam #2 Yeast (understand all about yeast – also, why are yeast a good model organism?) - The steps in the isolation of cell division cycle mutants in yeast - Pericentric inversion and paracentric inversion - know the consequences of single, double, triple, and four strand crossovers in inverted regions (or anywhere else) of heterozygous (or I assume a homozygous also) cross for both pericentric and paracentric inversions - know the answers to the above in terms of acentrics, dicentrics, duplications, and deficiencies and inverted sequences - know the meaning of and why each strand of DNA has polarity (also, think about polarities in replication, RNA, etc.) - The Hershey Chase experiment – know ALL about it - especially, what about the physical organization of bacteriophage T2 made it suitable for use in the experiment - The transformation experiment with S. pneumoniae - What are the effects of different chemicals which destroy specific things within the cell (proteins or DNA, etc.) (for example, the chemicals phenol, which destroys protein but not DNA, and NaOH (a strong alkali), which destroys both)? What do these results mean in regards to identifying the genetic material (remember, the purpose of the entire experiment was to determine what the genetic material was…I believe…?) - What is an auxotrophic mutant of E. coli? - How are alanine and methionine (both are amino acids) related to transcription/translation and the growth of an E. coli strain (i.e. why do you alanine and methionine, and what can you add to a cell media in order for the strain to grow in the absence of alanine and methionine?) Thing about how these facts could be applied to other questions. - Transcription and translation!!!! (All about them) – especially, what facts about transcription/translation are true for either prokaryotes or eukaryotes (think about statements that may be false or don’t apply to either prokaryotes or eukaryotes) - look at the practice test - GENE MAPPING (MAP DISTANCES, ETC.) KNOW EVERYTHING ABOUT THEM!!!! (NPD, PD, TETRATYPE, ETC.) (WHAT ARE THE IMPLICATIONS OF MISSING VITAMINS AND AMINO ACIDS AS IT PERTAINS TO THE RESULTS OF CROSSES THAT ARE GIVEN TO YOU) (ALSO, BE ABLE TO INTERPRET THE RESULTS OF ANY GIVEN CROSS AND WHAT THE RESULTS MEAN WITH REGARDS TO MAP DISTANCE AND GENE ORDER)
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(ALSO, THINK ABOUT LINKAGE RELATIONSHIP AND CENTROMERE DISTANCE) - RECOMBINATION!!!!- all about it - specifically, what are ALL the ways in which a recombination junction (also, look at Holliday Junction) can be resolved. (Be able to make drawings of these with flanking markers to distinguish the products) - REPLICATION FORKS!!!! - all about them - what are the implications of polarity as far as replication is concerned in a replication fork - what are the key players in replication forks (proteins? –ases? etc.) - on which chain of the fork will DNA synthesis occur in a direction toward the fork? - on which chain will the free -OH ends of the replicating DNA be oriented
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Genetics-Review for Exam 2 - Genetics Fall 2007 Things to...

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