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F07_202_25_Chap_2_6_12_DNA_Mapping-1

F07_202_25_Chap_2_6_12_DNA_Mapping-1 - Lecture 25 No...

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Lecture 25 No recitations Thanksgiving week HW (last assignment) Covering Lectures 22, 23, 25, 26 and 27 For Recitations: 11/28 and 11/29 AND: 12/5 and 12/6 Chapters 2, 6 and 12 Chap. 2: solved 2-4, 5,7,10-13,28,29 Chap. 6: solved 3, 25,30 Chap. 12: solved 1, 7, 15-17,22,26 NOTE: There will be exam questions on Lectures 28 and 29.
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How do we get clones for only the “expressed” genes? By “complementary DNA” (cDNA) cloning from mRNA RNAase added to degrade mRNA
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We switch now to the REST of Lecture 21  on gene structure and Eukaryotic gene expression NOTE you will need to know this for the final!
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Alternate promoters provide flexibility during development
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Alternate promoters may be selected depending upon which activator protein is present in a cell and bound to the enhancer
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Alternative splicing of the primary transcript increases protein diversity
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Unequal crossing over drives evolution
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Ig domains in many proteins
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Generation of diversity: Immunoglobulin G (IgG) molecule
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Fig. 21.22
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Exon duplication and subsequent rearrangement and alternate splicing create many different light chain proteins
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DNA bases are a Mendelian Trait!!
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