Practical 3 assessment questions 2017.docx - Practical 3...

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Practical 3 report The assessment for practical 3 is a written report in the format of extended answer questions. There are 3 questions in this report and each student should answer all 3 questions. Answers are expected to conform to the scientific writing guidelines on the Moodle page, including all citations (in-text and reference list) to be in the CSIRO referencing format. Guidelines on word count, submission, and getting-started hints can be found in the ‘Practical 3 Assessment Overview and Instructions’ document on Moodle. Students should ensure they read this document before beginning their report. 1. You have performed an experiment (your practical 3, Metabolism experiment). You wish to tell your scientist friend about this experiment so they can replicate it exactly. Write a methods section for this practical. This methods section should be brief. Do not write lists of materials and do not write methods in bullet-point or numbered form – write full sentences. You must describe how the activity of amylase was calculated in the germinating barley (section 2g). Describe the steps involved using words, not calculations. The methods should also explain (i) why a boiled control was used in 2f, and (ii) how you would correct the value of amylase activity for any of the stages if an achromic (Recommended word length 250-350 words). 10 germinating barley seeds were dried, weighed and crushed using a mortar and pestle in 10mL of buffer. This solution was filtered, and volume recorded in a 25mL measuring cylinder. A five-fold dilution was made up by pipetting 5mL into a measuring cylinder and then adding 20mL of buffer via auto pipette. A control was made up by adding 5mL of the amylase extract to a test tube and boiling for 10 minutes before being left to cool. This boiling of the amylase denatured the enzyme and could act as a control as it would not hydrolyze starch into maltose. One drop of iodine was placed into 22 wells on the ceramic test plates, 21 for experimental

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