exp_4_TA - Experiment 4: Experimenting with Concentrations...

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Experiment 4: Experimenting with Concentrations and Maximum Absorbencies of Chlorophyll-a Chem112-536 Dhara Bhakta, Rebecca Cherian, and Sean Collins (T.A.) Spring 2008 Introduction Chlorophyll, the green pigment found in plants, is essential to the plant’s health as it absorbs sunlight to provide energy in order for photosynthesis to take place. A spectrophotometer uses monochromatic light to probe a sample; as the monochromatic light passes through, the detector measures the decreasing light intensity which is generates a signal to be read as transmittance %. . In this lab, we are experimenting with chlorophyll-a to find the wavelength of maximum absorbance in a pure chlorophyll-a sample solution, the chlorophyll standard using a calibration curve, and the mg of chlorophyll-a per gram of a spinach leaf. Materials and Methods A Spectronic 20 spectrophotometer is set to a wavelength of 600 nm, and a transmittance of 0%. A blank cuvette ¾ full of 90% acetone is placed in the sample chamber and the transmittance is set to 100%. Then
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exp_4_TA - Experiment 4: Experimenting with Concentrations...

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