lambda assignment abdulrahman jijawi.docx - Restriction...

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Restriction Endonuclease Analysis of Lambda DNA Using NEB CutterStudent: Abdulrahman JijawiProfessor: Martina HausnerTA: Emma Brunhayne Course Code: BLG888Section 5Date Due: March 15th2018
TablesTable I. Fragment sizes generated by single digests of EcoRI, BamHI and HinDIII. This table shows the fragment sizes in base pairs generated by single digestion using different restriction sites. EcoRI(fragment bp)BamHI (fragment bp)HinDIII (fragment bp)212261684123130742172339416580467706682564365274361487856262322353055052027--564Table II. Fragment sizes generated by double digests of EcoRI+ BamHI and EcoRI+ HinDIII. 1
This table shows the fragment sizes in base pairs generated by double digestion using a combination of different restriction sites. EcoRI +BamHI(fragment bp)EcoRI + HinDIII(fragment bp)1572121226550551484669497337754268375835303530202732401904275217092564137518689471120831-564Table III. Number of restriction sites and fragments generated in each digest. 2
This table shows the number of restriction sites on the linear lambda DNA molecule and the number of fragments generated after single or double digestion. HinDIIIBamHIEcoRIEcoRI + BamHIEcoRI + HinDIIIRestrictionsites6551011Fragmentsgenerated7661112Figures3
Figure I. Single digestion restriction map for lambda DNA using EcoRI.This figure shows the restriction digestion map of lambda DNA using EcoRI restriction endonuclease. The numbers shown represent the size in base pairs of each fragment generated from each restriction site and the location of each restriction site on linear lambda DNA. 4
Figure II. Single digestion restriction map for lambda DNA using BamHI.This figure shows the restriction digestion map for lambda DNA using BamHI restriction endonuclease. The numbers shown represent the size in base pairs of each fragment generated from each restriction site and the location of each restriction site on linear lambda DNA. 5
Figure III. Double digestion restriction map of lambda DNA using EcoRI andBamHI.This figure shows the restriction map of lambda DNA using EcoRI andBamHI restriction endonucleases. The positions of EcoRI restriction sites relative to BamHI are donated by E and Brespectively, where the numbers shown are a representative for the location of the restriction site and the size in base pairs of the fragments generated.

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