final lab report.docx - Enzyme Function of Peroxidase with the effects of Extract Concentration pH Heat addition and Change in Temperature Kaelyn Keeley

final lab report.docx - Enzyme Function of Peroxidase with...

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Enzyme Function of Peroxidase with the effects of Extract Concentration, pH, Heat addition, and Change in Temperature Kaelyn Keeley Abstract The function of enzymes depends specifically on what conditions it is undergoing. An experiment has been conducted using Peroxidase as the enzyme. This enzyme has been put under different conditions to observe the absorbance rates at which they occur. The different temperatures and pH levels expressed different types of enzyme activity observed in this experiment. Introduction The usage of Enzymes is known to date all of the way back to prehistoric times, but the function of them wasn’t clear until the late nineteenth century or the first half of the twentieth (Talen-Peralse, 2015). Proteins that act as a catalyst of biochemical reactions to help with the function of living organisms are considered Enzymes. Enzymes play an important role in living organisms because they control reactions in these organisms by bringing substrates closer together and lowering the activation energy so the reaction can occur. A solution has to reach the top speed to then become saturated, meaning when all of the substrate molecules are occupied is when it tries to reach its maximum speed (Coleman, 2015). Enzymes are able to specifically recognize the substrate; the substrate then binds to the active site of the enzyme once it is recognized. In addition, all of the chemical reactions in a living system are established as metabolism (Coleman, 2015). A good example of an enzyme is Peroxidase. This enzyme is found in a eukaryotic organelle and can be also found in plant tissues. Peroxidase is better established as a heme-containing enzyme that is released by immune cells that are activated at sites of inflammation in human health (Panagopoulos,2015). In plants, peroxidase is involved a large range of physiological processes in the plant cycle. A function of peroxidase would be generating oxygen species and regulating levels of H 2 0 2 . Hydrogen peroxide is defined as a toxic by product of several reactions pertaining metabolism. (Coleman, 2015). To prevent biological or chemical attacks, these enzymes are able to build a wall or make the wall more flexible. From the beginning of the plants life to the end, the enzymes are present through everything (Passardi,2005). Under specific conditions Peroxidase is to be observed in this experiment to determine which be able to will produce the most enzyme activity. RH 2 +H 2 0 -> R + 2H 2 0 is the reaction after the peroxidase is removed from a turnip, this reaction will be investigated. Despite the reaction being almost impossible to see because of the reactions being colorless, Guaicol will be used as the hydrogen donor. The Guiacol is used as the reducing agent because it tends to have a change in color when losing hydrogens. This reaction is represented as Guiaiacol + H 2 0 2 -> Tetraguaiacol + 2 H 2 0(Coleman, 2015).
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