Unformatted text preview: metabolism. Why would you use the following steps in your procedure? a. Selection of ES cells with G418 b. Selection of ES cells with ganciclovir c. Southern blot using a blood sample from potential heterozygous mice (brown coat progeny) d. Mouse with Cre gene and heterogygous for GLUT2 crossed with a mouse containing the GLUT2 gene flanked with LoxP sites 4. What is the hallmark of retroviral DNA (key unique feature/sequence) and what is its purpose? 5. In outline, how does RNAi work? What are three limitations of this technique? 6. What would you expect to see when overexpressing a dominant negative mutant protein (as compared to wild type or loss of function mutations)? What is an advantage of using dominant negative mutants? Why are most dominant negative mutants of multi-subunit proteins (when would a single subunit dominant negative mutant be possible)?...
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This note was uploaded on 03/26/2008 for the course BIOBM 4320 taught by Professor Vogt,vm during the Spring '07 term at Cornell.
- Spring '07