Week2As - Week 2(Membranes Answers 1 True(T or False(F a T...

Info iconThis preview shows pages 1–2. Sign up to view the full content.

View Full Document Right Arrow Icon
Week 2 (Membranes) Answers: 1. True (T) or False (F) a. T b. F (this is a sign of an apoptotic cell) c. F (fatty acid chains determine the thickness AND fluidity) d. T e. F (the cytosol/inside is the reducing environment) 2. a. seven b. α helices; β barrels (composed of β sheets) would not give distinct hydrophobic and hydrophilic regions of the amino acid sequence. β barrels would have alternating hydrophobic and hydrophilic amino acids due to the nature of β sheet packing. c. See the plot above. This could be tested by the experiment outlined in Lecture 4, second page, second slide “Surface labeling of proteins to study their membrane topology.” Briefly, a membrane impermeable reagent is used to label lysine residues. The protein can be immuno-precipitated to purify the protein. Knowing the amino acid sequence, you can cut the protein into fragments at known locations. These fragments can be separated and individual fragments can be detected for the lysine label. 3.
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 2
This is the end of the preview. Sign up to access the rest of the document.

Page1 / 2

Week2As - Week 2(Membranes Answers 1 True(T or False(F a T...

This preview shows document pages 1 - 2. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online