15 - o 3 Insert new DNA(DNA ligase o Transfer plasmid to...

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Chapter 15 Bacterial Genetics o 1. Transformation Bacteria takes up DNA from environment and incorporated into itself o 2. Conjugation (may include a plasmid) 2 bacteria come together and form a conjugation bridge Sends DNA to one another Plasmid: vector o 3. Transduction (virus such as a bacteriophage) Bacteriophage: virus that infects bacteria DNA goes in and makes new viruses When DNA comes into the cell it may become embedded in the chromosome Genetic Engineering = Gene Splicing o Original gene splicing work done in bacteria Gene Splicing o 1. Must obtain a desirable gene Must know gene product Analyze to determine DNA sequence Look at protein and find sequence for amino acid Form or find DNA Could take artificial DNA and find where it matches Cut DNA using restriction enzymes o 2. Open a plasmid (restriction enzyme)
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Unformatted text preview: o 3. Insert new DNA (DNA ligase) o Transfer plasmid to bacteria o Find and select colonies making desired product •--Lots of epidermal growth factor in saliva • Discovery of Restriction Enzymes o Studying Haemophilus influenzae defense from bacteriophage Bacteria protected themselves by cutting up viral DNA before it inserted itself into the bacterial chromosome o Discovered bacterial enzyme that chops up viral DNA • Using a probe to find a gene o Find bacteria in a “library” containing a specific gene gene library: mixed collection of cells that have taken up cloned DNA o Need a probe for that gene Radioisotope-labeled piece of DNA Will base-pair with gene of interest • All cells have the same genes o Gene expression determines: Tissue type Cell functionality...
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