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discussions microbes.docx - DISCUSSIONS EXPERIMENT 1 :...

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DISCUSSIONSEXPERIMENT 1 : CULTURE TRANSFER TECHNIQUESIn this experiment, we were provided with three different cultures labelled “A”, “B”,and S. marcescensagar slant and each were transferred into a nutrient agar slant, nutrientagar deep, and nutrient broth. From Table 1 and Table 3, we can see that there are microorganisms growing insidethe nutrient broth and agar after 24 hours of incubation at 25oC in the incubator. Thepresence of microorganism in the nutrient broth can be seen as the broth turns cloudy fromcolourless solution. For agar slant, there are red pigmented growth on the surface of theagar where the bacteria was inoculated. Meanwhile on the agar deep, we can see that thebacteria grow only at the surface of the agar due to the need of oxygen for their survival. Forboth nutrient agar, there are only red pigmented growth without other colony ofmicroorganism which means that the bacteria had successfully being inoculated in a properaseptic techniques. After that, from Table 2 we can see that there are no growth of microorganismneither in the nutrient broth, nor the nutrient agar. The nutrient is still clear without anygrowth and there are no red pigmentation on the surface of the agar. This is because, cultureB that were inoculated was only a sterile tube of nutrient broth which means that thereshould not be any microorganism in the culture. After 24 hours of incubation, the resultshows that no microorganism present in the nutrient culture. This shows that the aseptictechniques that we use are good enough to prepare the pure culture. We had used sterile equipment and employed aseptic techniques when handlingbacterial cultures. By using aseptic techniques, it will minimizes the likelihood that bacterialcultures will be contaminated.There are several steps that are essentials during subculturing. Flaming theinoculating instrument prior to and aftereach inoculation are needed to completely free ofany living organisms that can be used to ensure bacterial growth and transfer it aseptically toanother container. Therefore, there will be no source of an external contaminant. Next, the
test tube cap was hold in the hand so that the test tube cap would not come in contact withany other contaminated surface and compromise the aseptic procedure. It also makes useasier to manage and transfer sample from one tube to the other and easier sterilize entryover flame. Then, to ensure that viable cells are transferred, we need to cool the inoculatinginstrument prior to obtaining the inoculum. When there is excess heat, it may killed thebacteria. After that, flaming the neck of the tubes immediately after uncapping and before

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Term
Fall
Professor
FADZLIE WONG
Tags
Bacteria, Agar plate, Nutrient Broth, nutrient agar, nutrient agar slant, agar slant

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