Page 22. A Researcher obtains a Soil Sample from a Garden. She uses a “Pinch” of this Soil to inoculate ~5.0 ml of Sodium Benzoate that has been added to each of five Test Tubes. She incubates these five inoculated Tubes for a Week at 25°C. Following Incubation all five Test Tubes appear Turbid. Our Researcher streaks a Loopful of turbid Sodium Benzoate from one of these five Tubes onto each of five Sodium Benzoate Plates. She incubates these five Sodium Benzoate Plates at 30°C for 48 Hours. After Incubation, Colonies of Bacteria appear on all of these Plates. Which of the Following represents the expected Appearance of the Colonies on these Plates and the expected Morphology and Gram Stain Results of the Bacteria taken from these Colonies?
3. Our Researcher uses this same Soil Sample to add a “Pinch” of Soil into a Centrifuge Tube containing ~5.0 ml of Water. She places this inoculated Tube in an 80°C Water Bath for 20 Minutes. After 20 Minutes she removes this Tube, allows it to cool, and vortexes it. She then uses a Sterile Loop to streak a Sample from this Tube onto five Nutrient Agar Plates. After Incubation at 30°C for 48 Hours, Colonies of Bacteria appear on all of these Plates. Which of the Following represents the Expected Results for Colony Morphology and Gram Stain Bacteria taken from these Colonies?