Exp. 1 Biosynthesis of Ethanol from Molasses Lab Report - Experiment#1 Biosynthesis of Ethanol from Molasses Kimberly Huynh Dr Truttmann Chem 80

Exp. 1 Biosynthesis of Ethanol from Molasses Lab...

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Experiment #1: Biosynthesis of Ethanol fromMolassesKimberly HuynhDr. TruttmannChem 80 Section #4186904/11/18
INTRODUCTION:The purpose of this experiment was to utilize a green chemistry approach to synthesize ethanol through the fermentation of sugars found in blackstrap molasses. The sugars then undergo fermentation using water and baker’s yeast, which includes two enzymes, sucrase and zymase. Sucrase helps convert sucrose into fructose and zymase converts fructose and glucose into ethanol and carbon dioxide, as shown in the two balanced equations below. The reaction was then vapor locked with lime water (Ca(OH)2) solution to keep the ethanol from oxidizing into acetic acid. Ethanol was then separated and purified from the ethanol-water mixture by simple distillation and fractional distillation. Simple distillation is a method used to purify a compound that’s practically pure with less than 10% purity or when the boiling points of the liquids in the mixture have a difference of at least 100°C. On the other hand, fraction distillation is a method used to separate two liquids with similar boiling points. In this type of distillation,a fractionating column is used and allows the mixture to undergo several vapori-zation/condensation cycles. Within the column, the composition of the vapor is continuously enriched with the lower boiling point. After the distillations were complete, the purity of the ethanol was tested by ignition tests. Since ethanol is a highly flammableorganic compound, it should ignite and burn with a clear blue flame when ignition tests are performed. sucraseC12H22O11(aq)+H2O(l)C6H12O6(aq)+C6H12O6(aq)(1)sucrosewater catalystfructose glucose zymaseC6H12O6(aq)+C6H12O6(aq)4C2H5OH(aq)+4CO2(g)(2)fructoseglucose catalystethanol carbon dioxide DATA:Table 1: Experimental Data for Simple Distillation of EthanolFractionApprox.Volume(mL)CollectionTemperatures(°C)Mass of1.000 mLaliquot (g)ColorofFlameDuration ofFlame(s)1579.0-85.50.7843clearblue8.102587.10.8185clearblue6.87
3588.90.8717clearblue7.084584.90.8781clearblue9.005583.50.8545clearblue8.016583.00.7875clearblue8.37First drop of distillate for fractions 2-6 were not taken.

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