Fast Plants Lab

Fast Plants Lab - Alyona Avdonina Fast Plant Research...

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Alyona Avdonina Fast Plant Research Project Dan Gates Effects of Gibberellic Acid on Development of Fast Plants Introduction : Plant growth is affected by a variety of factors. Internally, it is regulated by natural chemicals called hormones. By standard definition, as cited from Meriam-Webster’s Medical dictionary, plant hormones are “an organic substance other than a nutrient that in minute amounts modifies a plant physiological process, especially when active somewhere other than the site of production”. Plant hormones,also called phytochromes, are responsible for the growth and differentiation of the plant producing them. There are several different classes of phytochromes, one of which is the gibberellins. This group of hormones is responsible for several developmental functions. Gibberellins break the dormancy of a seed, stimulates cell elongation and bolting and causes seedless fruit development (“Gibberelins”). Under regular circumstances, the plant itself will synthesize the hormones necessary for regular plant growth. A failure to do so will result in the absence of the physical phenomena induced by that hormone. Experimental overview: In order to investigate the effects of gibberellin on plants, a mutant strain of Brassica (Wisconsin Fast Plants) lacking in the capacity to efficiently synthesize gibberellins was obtained. This strain is deficient in height and will grow to express a rosette phenotype. In order to determine the role of gibberellic acid, a gibberellin, on plant development, the acid was topically administered to both a standard (2 cells, 2 plants each) and dwarf (rosette) (2 cells, 2 plants each) form of Brassica and the results were then compared to a control, which, again, consisted of two sets each phenotype, four plants each. All plants were planted in Styrofoam cells equipped with a wick and placed on a water tray for self-watering. After allowing the plants 7 days to germinate, they were checked regularly to record height and apply gibberellic acid to the leaves of the experimental plant group. Hypothesis : We hypothesized that a regular application of gibberellic acid to dwarf plants would at least partially restore its normal elongation function, allowing the dwarfs to exhibit growth patterns akin to that of the control standard group, outgrowing the control dwarfs. We also hypothesized that extra gibberellin, when applied to the experimental standard group,
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This note was uploaded on 03/27/2008 for the course BIOL 212 taught by Professor Shen during the Spring '08 term at Iowa State.

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Fast Plants Lab - Alyona Avdonina Fast Plant Research...

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