Variables Affecting Reaction Rate.docx

Variables Affecting Reaction Rate.docx - Mia Ruiz Variables...

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Mia Ruiz Variables Affecting Reaction Rate In Requirements of BIOL 1406 – R 10/26/18
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Mia Ruiz BIOL 1406-R Variables Affecting Reaction Rate ABSTRACT Reaction are observed at different enzyme concentrations, substrate concentrations, temperatures, and inhibitor concentrations to determine how these variables affect the reaction rate. The reactions for enzymes were measured in solutions of high, medium, and low concentrations. Turnip extract was used as a source of the enzyme called peroxidase. Guaiacol was used in the enzyme solutions in its oxidized state along with a spectrophotometer to quantify the reaction rate in an absorbance vs. time graph. The absorbance was taken of each concentration every 20 seconds for 180 seconds. The high enzyme concentration provided the highest rate of reaction while the medium and low concentrations provided about the same rate. The reactions for substrates were measured in solutions of high, medium and low concentrations as well. Peroxide was the substrate used. Guaiacol was also used in the substrate solutions to quantify the reaction rate via spectrophotometer. The absorbance was taken of each concentration every 2 seconds for 180 seconds. The medium concentration of substrate provided for the fastest rate of reaction, the high was the next fastest, while the low was the slowest. The reactions for temperature were measured at 4 ° C, 37 ° C, and 60 ° C. The reaction at 4 ° C was the slowest while the reaction at 60 ° C was the fastest. The reactions for inhibitor were measured at 0 drops, 1 drop, and 5 drops of inhibitor in an enzyme solution. The inhibitor used was hydroxylamine. The reaction at 0 drops was the fastest while the reaction with 5 drops was the slowest. INTRODUCTION Enzymes are proteins and they are catalysts in that they lower the amount of activation energy needed for a reaction to take place, or for a product to form in a reaction. They work in conjunction with a reactant called a substrate. The substrate attaches to a spot on the enzyme called the active site. If there is high substrate concentration then increasing the concentration of
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