pre lab 17 .pdf - Lenny B ACHM 125 Experiment 17 Buffer...

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10/17/18 Lenny B. ACHM 125 Experiment 17: Buffer Solutions Objective: To find the pKa of acetic acid using different methods and to determine if an acetic acid / sodium acetate buffer solution is able to resist changes in pH. Method 1 : Use the formula for Ka and convert it to pKa to find the pKa of acetic acid u to determine if an acetic acid / sodium acetate buffer solution is able to resist changes in pH. Method 2 :Use the Henderson-Hasselbalch equation for acetic acid to find the pKa of acetic acid to determine if an acetic acid / sodium acetate buffer solution is able to resist changes in pH Hypothesis: Method 1: Method 2: Variables: Method 1: Independent - Acetic acid Dependent - Buffer solution Method 2: Independent- Dependent- Experimental Outline: Determining the Concentration of Acetic Acid (Make 150 mL of approximately 0.1 M NaOH from 5.0 M NaOH and standardize it against KHP over three trials. 1. Before titrating acetic acid, standardize the base. 2. Dilute the appropriate amount of 5.0 M NaOH to make 150 mL of 0.1 M NaOH 3. Measure the 5.0 M NaOH using a graduated cylinder and a 250 mL Flask to make the solution 4. Standardize 1.0M NaOH using KHP. Make 3 trials
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