Lab 1_ Diffusion and Osmosis.docx - Mackenzy Garden Mrs Papia AP Biology 15 November 2018 Lab 1 Diffusion Observation The concentration of the sucrose

Lab 1_ Diffusion and Osmosis.docx - Mackenzy Garden Mrs...

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Mackenzy Garden Mrs. Papia AP Biology 15 November 2018 Lab 1: Diffusion Observation : The concentration of the sucrose is higher in the dialysis bags than in the surrounding water. Question : Will the mass of the liquid on the inside of the dialysis bag change? Hypothesis : It will be proven that diffusion between solutions of different concentrations will occur until equilibrium is reached. Prediction : When the bag is placed in the container of water, slowly the molecules of sucrose will move through the miniscule holes of the bag into the water. Materials : Dialysis tubing Water String 15% glucose/1% starch 250mL beaker Distilled water Lugol’s solution Testape 0.2 M sucrose 0.4 M sucrose
0.6 M sucrose 0.8 M sucrose 1.0 M sucrose Scale Stop watch Procedure : 1. Obtain a 30-cm piece of 2.5-cm dialysis tubing that has been soaking in water. Tie off one end of the tubing to form a bag. To open the other end of the bag, rub the end between your fingers until the edges separate. 2. Test the 15% glucose/1% starch solution for the presence of glucose. Your teacher may have you do a Benedict’s test or use glucose Testape or Clinistix. Record the results in Table 1.1. 3. Place 15 mL of 5% glucose/1% starch solution in the bag. Tie off the other end of the bag, leaving sufficient space for the expansion of the contents in the bag. Record the color of the solution in Table 1.1.

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