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Fed-BatchNutrients are continuously or semi-continuously fed, while effluent is removed discontinuously.- overcome substrate inhibition or catabolite repression by intermittent feeding of substrate by maintaining low substrate concentration.for production of secondary metabolites e.g. antibiotics, lactic acid, E. Colimaking proteins from recombinant DNA technology.
Fed-Batch Analysis of fed-batch with substrate continuously fed and no output: t=0, V0=0, F is constant.At quasi steady state, S added=S consumed, X, S, P concentrations are constant.Volume:Cell mass balance: