BIO291-Ch. 23 Lab Report Enzyme Activity_Chelsea Aguirre.doc - LABORATORY REPORT Activity 1 Enzyme Activity Name Chelsea Aguirre Instructor Charles Chau

BIO291-Ch. 23 Lab Report Enzyme Activity_Chelsea...

  • University of Phoenix
  • BIO 291
  • Lab Report
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LABORATORY REPORT Activity 1: Enzyme Activity Name: Chelsea Aguirre Instructor: Charles Chau Date: December 9, 2018 PREDICTIONS 1. Sucrase will have the greatest activity at: pH9 2. Sucrase will have the greatest activity at: 70°C (158°F) 3. Sucrase activity: Increases with increasing concentration until a plateau is reached. MATERIALS AND METHODS Effect of pH on Enzyme Activity 1. Dependent Variable: Amount of product (glucose and fructose) produced. 2. Independent Variable: pH. 3. Controlled Variables: Temperature, amount of substrate (sucrose) present, sucrase + sucrose incubation time. Effect of Temperature on Enzyme Activity 1. Dependent Variable: Amount of product (glucose and fructose) produced. 2. Independent Variable: Temperature. 3. Controlled Variables: pH, amount of substrate (sucrose) present, sucrase + sucrose incubation time. Effect of Substrate Concentration on Enzyme Activity 1. Dependent Variable: Amount of product (glucose and fructose) produced. 2. Independent Variable: Amount of substrate (sucrose) present. 3. Controlled Variables: Temperature, pH, sucrase + sucrose incubation time.
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4. Describe what is measured as an indicator of sucrase activity and why this is an indicator of sucrase activity. Optimal density is measured so the amount of glucose and fructose produced is measured. 5. Explain why denatured sucrase was used as a control. Denatured sucrose was used as a control because it will not have a high activity rate like other sucrose. 6. Addition of DNS at the end of the incubation period stopped the reaction by denaturing sucrase. Explain why it is important to denature sucrase before measuring product concentration.
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