Spring '07 exam 3

Spring '07 exam 3 - Exam 3 BBMB 405 April 13, 2007 Name...

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Unformatted text preview: Exam 3 BBMB 405 April 13, 2007 Name ____ l. (5 pts) The function of the ryanodine receptor (calcium channel protein) in contraction of mammalian skeletal muscle proteins is to rapidly M $42» We 2. (9 pts) During a contractile event, the “a ‘ cl and ti ‘ “3% (names of sarcomeric bands or zones) shorten (narrow), whereas the AM (band) does not change in length. 3. (6 pts) The l M ~C— (subunit of troponin, TN) binds Ca2+ ions reversibly during contraction/relaxation; whereas the TN - ; (subunit of troponin, TN) binds to actin in relaxed muscle, but not during a contractile event. 4. (3 pts) What are the three enzymatic activities of reverse transcriptase? a. WWW 2W) 117sz y@¢;fi (417% 4.1.9:; flaggWJ/m- c. M733: 5. (5 pts) Why do oligonucleotides containing Shine—Dalgarno sequences inhibit translation in prokaryotes? Why don’t they do so in eukaryotes? quldflww . _ ct. mew WWW-#4214) 7f; W‘TR'UQ- «Wt-J W "‘ m . 41A) Mug“ . 6. (4 pts) Which ocess requires m high energy p osphate bond energy per amino ac1d incorporated into globin in an active reticulocyte: Protein synthesis or synthesis of mRNA for globin (per amino acid basis)? Assume the amino acids are intracellular. Wm Justify your answer. W WWflW/g a ’V 19/5 4% A .q . 7. (4 pts) The size of individual molecules of mRNA in both E. coli and rats determine the size of protein synthesized from the RNA molecules. 7%. Yes or N Justify your answer. MEN)?» 15% max/a ftfii 4;, Wm; Lag/2%“; W 74;» awed. ’ 8. (4 pts) How do the polypeptide products produced in the presence of (a) streptomycin and (b) puromycin differ from polypeptides synthesized in the absence of these inhibitors? a. Streptomycin S 0L b. Puromycin P W ‘ ' / 9. 6 p ) atch e fem right co umn With t e type 0 DNA helix in the left column. (May be more than one answer per blank.) 21) A—DNA é 7 (l) Phosphates in the backbone zigzag along the helix. b) B—DNA 3. 41 7 (2) Formation is favored sequences of alternating purine C) Z-DNA (, 2. and pyrimidines. (3) Has a relatively wide and deep major groove. (4) Has a right—handed helix. (5) Has 10.4 base pairs per turn. (6) Has a structure similar to that of double—strand RNA. (7) Strands in the helix have opposite polarities. lO. (3 pts) Eukaryotic DNA can be highly methylated at the C-5 position of cytosine. The degree of methylation is correlated inverSely with gene expression. Although the exact role of C-5 methylation in gene expression is not known, it is known that these C—S—methylated cytosines can cause mutations. How? [Hintz Deamination?] Q~5’ Mawffim WWaé, java/7’ W 6%. cps/Mac .197- MM MW“ twig m7” ‘1'“... ll. (6 pts) Match the descriptions in the right column with the apprOpriate eukaryotic DNA— dependent RNA polymerase in the left column. Q a) RNA polymeraseI () L 8 (l) is located in the nucleolus b) RNA polymerase 11 3k, 3, 2 8,2 [0 (2) is located in the nucleoplasm c) RNA polymerase III a, 5 g f (3) makes mRNA precursors (4) makes tRNA precursors (5) makes SS rRNA (6) makes 18$, 5.8S, and 285 rRNA precursors (7) is strongly inhibited by OL-amanitin (8) synthesizes RNA in the 5’ 9 3’ direction (9) is composed of several subunits (10) has a subunit with repeated amino acid sequences subject to phosphorylation 12. (4 pts) The template strand of DNA known to encode the N-terminal region of an E. coli protein has the following nucleotide sequence: GTAGCGTTCCATCAGATTT. Give the sequence for the first four amino acids of the protein. See genetic code on last page. Show work. 5’ ’ Twyla 4}; (Tea Uc ngIgG @373 «ram c truce-c mac GUI? 4.06 umf’ I —— I l / “1w 4A Mi m WALL. fMDhCVZJ: (bani/“7's l3. (3 pts) Differentiate between DNA synthesis that is catalyzed by DNA telomerase and by DNA polymerase in your skin. f-D/UA WM’.‘ WEN/90°:— 4~ ZE’VW‘M (921% Wma cam 14. (5 pts) RNA polymerase is incubated with a DNA template, all the necessary transcription factors, and the substrates ATP, UTP, GTP, and CTP, which are labeled with ‘80 in the B— phosphate group and 14C in the ribose. Will 180 and 14C be incorporated into the RNA that is synthesized? Jugify your angwflg. “0 I 0"P ‘0 7: '9‘ P-D~Zflz 604—2 25 5:"? 23 lg +P ‘(k \ ,P [p % ((9 PF 072/ 0< 15. (4 pts) Dr. Turner, a well-known pioneer in the study of lactation physiology at the University of Missouri, reported the weight ratio of DNA to RNA in lactating and nonlactating mammary glands of several vertebrate animal species. What information can one learn by comparing the DNA to RNA ratios of (a) lactating and nonlactating animals and (b) different lactating animals of the same species. (a) U“ T Ia/DA/fl lav/em _ , ’ W (b) W \ /w ’DAle 2% lam 16. (4 pts) Extensive base pairing between the ribosomes and mRNA of prokaryotes and eucaryotes is necessary for high fidelity in transition. i‘:e2§:4 Justify your answer. True or False l7. (5 pts) How many grams of glucose do you need to generate sufficient ATP to resynthesize 300 grams of body protein per day from intracellular amino acids. Assume all necessary ATP is generated from glucose oxidation and the following relationships: 36 ~ P per glucose oxidized to C02, molecular weight of glucose is 180, and of an average amino acid is 100 grams per mole. grams glucose per day. Show calculations. /Zfl?4) X baa/€26- X44173 K [M44X3lmaflf We Bea/P Mata Wag“ 403 (r 18. (4 pts) Assume that the E. coli genome contains 4.64 x 106 base pairs. Assume the average length of the Okazaki fragment is 2,000 nucleotides, how many Okazaki fragments will be produced during the replication of one entire E. coli genome? Show calculations. 4; LILré¢X 10 4% >( 5%??? :: Jim/(33% 3,320 l9. (4 pts) How does the presence of the nonhydrolyzable GTP analog shown below affect in vitro protein synthesis? 0 o n u GMP—O—Ef—NH—li—O' o’ 0' G mnesine—S'fld—imidoytriphosphate (CD PM?) adéjtfiufi I a 20. (4 pts) A eukaryotic ribosome contains 4 different rRNA molecules and ~82 different proteins. Why does a cell contain many more copies of the rRNA genes than of the ribosomal protein genes? ARM/9W >Wm¢f4®¢ WW W 424 21. (4 pts) Answer True or False: a. An All“ base pair and a C/G base pair have about the same physical dimens‘ons 2%. b. If GGGGCCCC represents the sequence of bases in one strand of a double-stranded DNA, then the complementary strand must have the sequence CCCCGGGG c. mRNA is single—stranded ; . d. rRNA and tRN A are devoid of unmodified nucleosides ./ 23. (4 pts) How are the 5’ and 3’ ends of eukaryotic mRNA transcripts typically modified? 5/M. WW 3 'eAWMW The Standard Genetic Code UUU Phe UCU Ser UAU Tyr UGU Cys UUC Phe UCC Ser UAC Tyr UGC Cys UUA Leu UCA Ser UAA Slop UGA Slop UUG Leu UCG Ser UAG Slop UGG Trp CUU Leu CCU Pro CAU His CGU Arg CUC Leu 000 Pro CAC His 060 Arg CUA Leu CCA Pro CAA Gin CGA Arg iCUG Leu 006 Pro CAG Gin CGG Arg AUU lle ACU Thr AAU Asn AGU Ser AUC lle ACC Thr AAC Asn AGC Ser AUA lle ACA Thr AAA Lys AGA Arg AUG Met ACG Thr AAG Lys AG G Arg GUU Val GCU Ala GAU Asp GGU Gly GUC Val GCC Ala GAC Asp (360 Gly GUA Val GCA Ala (3AA Glu ' GGA Gly GUG Val GCG Ala GAG Glu GGG Gly AUG is pan of the initiation signal‘ as well as being the codon for internal methionine. 1 Symbols for Amino Acids A Ala Alanine . M Met Methionine B Asx Asparagine or aspartic acid N Asn Asparagine C Cys Cysteine P Pro Praline D Asp Aspartic acid 0 Gln Glutamine E Glu Glutamic acid H Arg Arginlne F Phe Phenylalanine S Ser Serine G Gly Glycine T Thr Threonlne H His Hisiidine V Val Valine I lie lsoleucine W Trp Tryptophan K Lys Lysine Y Tyr Tyrosine L Leu Leucine Z Glx Glutamine or gluiamic écid ...
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Spring '07 exam 3 - Exam 3 BBMB 405 April 13, 2007 Name...

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