Absorbance Spectroscopy and Beer's Law.docx - Brandon...

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Brandon Comiskey-Lucero Raymond Chem121L Sec 8 December 3, 2018 Absorbance Spectroscopy and Beer’s Law Introduction In this experiment, we focused on understanding the absorbance of molecules and ions and about Beer’s Law. To do this we began by making sample solutions of known Molarities of Cupric Sulfate Pentahydrate. We did this so that we could determine a linear trendline to use to calculate the Molarity of any other solutions we were given and more specifically the unknown we were given, just by knowing the absorbance in the spectrometer. Experimental Data To start off the experiment we had to calculate how much Cupric Sulfate Pentahydrate (CuSO4 ?5H2O) we would need to create a 0.1M solution that is 50 mL. We calculated that we would need 1.244 grams of the solute so we went on to weigh this amount out. We obtained approximately 1.243 grams of our CuSO4 ?5H2O in this step. Next we mixed it with water to create a 50 mL solution. Next we took a sample of this solution and put it in a cuvette. We moved on to begin
creating the dilutions. To do this, we took 25 mL of the previous solution and mixed it with 25 mL of water. We did this 4 more times until we had solutions of 5 different Molarities. The 5 Molarities we had are shown below as well as the unknown sample and a water sample.

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