Final Exam 2

Final Exam 2 - BBMB 495 Final Examination Open Book Part...

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Unformatted text preview: BBMB 495 Final Examination Open Book Part (120 pts) May 3, 2997 Name l. (5 pts) OL-32P-dATP can be used as a labeled precursor for assay of DNA synthesis, whereas y—sz-dTTP cannot be used as a precursor for the assay. . Why? A F ’0 Tme or False o % . “3' l‘f‘FeflQ—JQ % nmwl 2. (1 pt) Name an example in nature where RNA serves as both genetic material and mRNA. 3. (3 pts) Does all nucleic acid in an animal or plant cell exist as a nucleoprotein? W0 . Justify your answer. Yes or No aim/AW! 42 6M’ W ' 4. (4 pts) Do human liver cells contain both linear and circular DNA mol . Defend your answer. Yes No ‘ .. / . 5. (5 pts) You have a column containing a cellulose to which polythymidylate (poly T) fragments have been attached covalently. Could you use this column for separation of a specific class of nucleic acids from a rat liVer cell extract? %J:Y S or NO . Explain. V y wfiM/Q' M 6’ W gm PN/gw WW W l 1 6. (4 pts) Ovalbumin, a major protein of egg white, can be synthesized in a transformed E. coli. The gene for ovalbumin contains seven introns. To generate the transgenic E. coli, would you use a cDNA for ovalbumin or the genomic DNA (naturall occurrinU in DNA) for ovalbumin to transform the E. coli? gLD 57» ,Why? a’DMfiMfla W, 7. (4 pts) Human liver can convert the acetic acid in vinegar to glucose with a net yield. I W12 Defend your answer. True or False 8. (5 pts) When you begin to fast, describe why hormone—sensitive lipase (HSL) is necessary for the increased rates of synthesizing ketone bodies such as beta—hydroxybutyrate. (EH-3 9. (8 pts) Schematicall illustrate the sequence of biochemical events between the releaSe of epinephrine into the bloodstream and the activation of glycogen phosphorylase. 10. (5 pts) Discuss the role of ubiquitin and proteasomes in regulating the concentration of ornithine decarboxylase in your liver cells. (9% {Ma 25 A) I A . Q 4, MJ W‘ > W 11. (4 pts) When mRNA specifying a secretory protein was translated Wnce of endoplasmic reticulum, the protein product was longer than the form secreted by living cells. Explain why. 5 ‘ ’ wflé, W I 12. (6 pts) Is lipoprotein lipase (LPL) necessary for the deposition of dietary tripalmitin (a triacyl glycerol) in adipose tissue of humans? Explain. 3 r 4 4 PC / b?% I 7724 LPL W Ami’kmtr Qofiréwkié“ Fa «h. “9 Camum W. 13. (6 pts) Human brain does not directly oxidize long—chain fatty acids to CO; for ATP generation. Explain how the liver of a severely fasted person allows fatty acids derived fr m adipose tissue to indirectly provide oxidizable fuel molecules to the brain for ATP generation. M m 7 da // 2 l< )3 W 7264? F74 , (:0 G v ' [41% K6 3 _ Yes r No DJ Q l4. (3 pts) Human patients with a deficiency of phenylalanine hydroxylaSe in tissues have a lower dietary requirement for phenylalanine than do normal patients. Why? Bahaflgbw, ; UM¢W 1L 3 Uaaéa 4M 12% M” t“ ’644/ La in your pet dog. Why? , MA ‘I )Q l . (3 pts) Indicate the role of hospholi se in product' 11 of pros glandin E2 in your body. % PWEAM’ (was Wm t W we WWOWWMMWW l7. (5 pts) SuppOSe the extracellular lecithin-cholesterol acyltransferase (LCAT) was deficient in a person. Would you expect the person to be at greater risk for heart disease? Why? Yes rNo 1 18. (5 pts) Suppose you have incubated slices of bovine mammary gland with 2—14C—malonate, unlabeled acetate, and other “goodies” in media so that de novo fatty acid synthesis occurred Which carbon atoms within a synthesized palmitate (CI—16) molecule would you expect to find the 14C? Explain your answer. 0\ *5 30 '.0 :C~C—O\SM +flcm 3 -clv[5-;/V£~CA§[Z O 19. If you consumed polyalanine and you consumed food in excess of your energy needs, you M would deposit some of the carbon in this polyalanine as triacylglycerols in your adipose [at Q g tissue. Outline the metabolic reactions in your small intestine, liver, and adipose tissue that n) allow for this transfer of dietary polyalanine to body fat. a. (3 pts) Intestinal tract: T; ————-3 W b. (3 pts) LjV_6[: V C- (3 PtS) Adi ose tissue: L D L T” a: ._ [(Hr fiFfi—fl 1/7541»; VLDL LP W 20. (6 pts) Match the following structure with the appropriate description. g Transfers electrons to PS I A. P680 AEi Transfers electron to cytochrome complex B. P700 Q Mediates transfer of electrons from ferredoxin to NADP+ C. oxygen evolving complex L Highly oxidizing complex associated with PS H D. Fd—NADP+ reductasc 1. Reaction center for PS I E‘ plastocyanin A Reaction center for PS H F. plastoguinol 21. (4 pts) Would you expect the rate of erythrose-4—phosphate metabolism to be greater in normal or in cancerous pancreatic tissue? Justify your anSWer. E'4~FA€M7MMT ‘ 22. (2 pts) A salvage enzyme exists to add uracil to PRPP, but there is not a similar one for thymine. Can you form a logical explanation for this? If my Mg MM PRPP ("i 55/ Milt MW 13% whmuc/Eizl (3/2th (Elli We 0/ WW0 (N l’tl’i‘h is SWIM/fed 23. (2 pts) Listed below are two reactions in the urea cycle. Which enzyme in the de novo IMP synthesis pathway catalyzes a similar reaction? COO W H COO’" NH2 ZHN \(0 +H2N #V +H2N # NH H NH COO~ ' 00" + J :2. + . v . . . . - . . .. y, l \‘C'H \ H , a \ H ‘ \"HH +H3N coo- *H3N coo ATP AMP +H3N coo + 3N COO" ‘ooc Citrulline Aspartate PPi Argininosuccinate Arglnlne Fumarate 5143—54123 Swill/Last: QV‘iQi/Jffid9W/MICOMid 24. (18 pts) State the principal organ or tissue of a lactating human that conducts the following processes: a. Synthesis of pepsinogen W . - W b. Synthesis of daughter strands of DNA 6 k( 2] 52.1142 W): arr M c. Conjugation of taurine to cholic acid Din/V‘e/‘L/ d. Synthesis of ribonuclease for secretion WW 6. Conversion of palmitate to acetoacetate f. Incorporation of cholesteryl esters into chylomicrons 5 MW g. Conversion of VLDL to LDL ' I r‘é . W h. Synthesis of lysine from glucosc and amrnonia%)_uilzg_—é(£',£Qv/&M:a i. Conversion of carbamoyl phosphate to urea _ j. Incorporation of 0L—glyccrol phosphate into triacylglycerolm ; 72¢“ k. Synthesis of galactose from glucose W W 1. Conversion of alanine to glycogen ) \ m. Synthesis of dipalmitoyl lecithin for extracellular use we fit? n. Conversion of glucose to oleic acidMézépa é 0. Conversion of glucose to linoleic acid Mil % p. Synthesis of nascent HDL it 4-2 2 q. Target tissue for glucagon M r. Total absence of DNA repair activity 6 g , HAVE A GREAT SUMMERH CI ...
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Final Exam 2 - BBMB 495 Final Examination Open Book Part...

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