26.rtf - Name Date A B C D E A B C D E 1.RNA polymerase...

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Name: __________________________ Date: _____________ 1.RNA polymerase: A) binds tightly to a region of DNA thousands of base pairs away from the DNA to be transcribed. B) can synthesize RNA chains without a primer. C) has a subunit called (lambda), which acts as a proofreading ribonuclease. D) separates DNA strands throughout a long region of DNA (up to thousands of base pairs), then copies one of them. E) synthesizes RNA chains in the 3 ' 5 ' direction. 2.Which statement about E. coli RNA polymerase is FALSE? A) Core enzyme selectively binds promoter regions, but cannot initiate synthesis without a sigma factor. B) RNA polymerase holoenzyme has several subunits. C) RNA produced by this enzyme will be completely complementary to the DNA template. D) The enzyme adds nucleotides to the 3 ' end of the growing RNA chain. E) The enzyme cannot synthesize RNA in the absence of DNA. 3.Which statement about E. coli RNA polymerase (core enzyme) is FALSE? 4.Which property does NOT apply to RNA polymerase from E. coli (core enzyme alone)? C) It produces an RNA polymer that begins with a 5 ' -triphosphate. D) It recognizes specific start signals in DNA. E) It requires all four ribonucleoside triphosphates and a DNA template. 5.The sigma factor of E. coli RNA polymerase: Page 1
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the core enzyme. 6.After binding by E. coli RNA polymerase, the CORRECT order of events for transcription initiation is: A) closed complex formation, open complex formation, promoter clearance, start of RNA synthesis. B) closed complex formation, open complex formation, start of RNA synthesis, promoter clearance. C) open complex formation, closed complex formation, start of RNA synthesis, promoter clearance. D) start of RNA synthesis, closed complex formation, open complex formation, promoter clearance. E) start of RNA synthesis, open complex formation, closed complex formation, promoter clearance.
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