Unit 1 - Bound Resiudes 2019 Part 4.doc

Unit 1 - Bound Resiudes 2019 Part 4.doc - NRS 412...

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NRS 412 - Soil-Water Chemistry Spring 2019 Part 4 - An Unextractable Problem You carried out the experiment and more than 90% of the 13 C was found associated with the humic acid fraction of soil organic matter. You set out to purify the 13 C-labeled humic acid so that you can do structural characterization of the material, perhaps using that nuclear magnetic resonance spectrometer that the guys down the hall just built using an old radio transmitter and a magnet from a junked generator. You figure that the same methods that work for purifying things like proteins and DNA should work for humic acid. “They are all organic molecules. Proteins have acidic groups in them, and there’s nucleic acids in DNA. I’ve seen pictures of a humic acid molecule on the interwebs… Yeah. Not a problem.” Drawing on your previous experience as a biochemistry lab technician, you decide to use gel permeation chromatography-mass spectrometry (GPC-MS) to purify your humic acid-PCA-bound-residue-thing. The column is good for
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  • Spring '19
  • JoseAmador
  • Class Work, Molecule, Atomic mass unit, Humus

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