Application Note 337 - Setup of a Microbial Hyaluronic Acid Production Process Using the BioFlo 120 - APPLICATION NOTE No 337 I February 2017 Setup of

Application Note 337 - Setup of a Microbial Hyaluronic Acid Production Process Using the BioFlo 120

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Setup of a Microbial Hyaluronic Acid Production Process Using the BioFlo ® 120 Bioprocess Control Station Geetanjali Wakade 1 , Tushar Sabale 1 , Mandar Deshpande 1 , Shruti Panchwagh 1 , Ulrike Becken 2 1 Praj Industries Limited, Pune, India; 2 Eppendorf Bioprocess Center, Juelich, Germany Contact: [email protected] APPLICATION NOTE No. 337 I February 2017 Abstract Achieving optimal process conditions for microbial growth and production of a desired bioproduct needs hardware and software to monitor and control the bioprocess parameters, including the pH, temperature, and agitation of the fermentation broth. Intuitive bioprocess software and an easy-to-use bioprocess controller can accelerate process development, because they reduce the time needed to train staff, prepare the system, and start the process run. Researchers at Praj Industries, India used the BioFlo 120 bioprocess control station to produce hyaluronic acid (HA) in Streptococcus zooepidemicus at bench scale. In this application note we describe how they set up, monitored, and controlled critical process parameters, and thus present an impression of the controller’s integrated, touchscreen- accessible software. Introduction HA is a polymer composed of repeating disaccharide units of β-1,3-N-acetyl glucosamine and β-1,4-glucuronic acid. High concentrations of HA are present in the skin, the vitreous humor, and the umbilical cord. HA is also a component of the capsules of certain microbial strains. It is industrially produced for a variety of biomedical applications and cosmetics, mainly by microbial fermentation of natural producers like Streptococcus spec. or recombinant bacterial strains. Praj Industries Limited based in Pune, India, offers innovative solutions for beverage alcohol and bioethanol plants, brewery, water and wastewater treatment plants, critical process equipment and systems, and bioproducts. They tested the BioFlo 120 bioprocess control station (Fig. 1) for HA production in Streptococcus zooepidemicus , and in this application note describe the setup and operation of the fermentation process.
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