Practical 1 Review Micro.pdf

Practical 1 Review Micro.pdf - EXERCIESE 1 Microscopy and...

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EXERCIESE 1: Microscopy and Morphology/Arrangement of Bacterial Cells Name of the compound light microscope used in lab: Brightfield Compound Light Microscope 1. Ocular Lens (10x) 2. Revolving Nosepiece (above objective lens) 3. Objective Lens 4x 10x 40x 100x Oil Immersion used with 100x because it has the same refractive index as glass, diminishes the loss of refracted light and improves resolution 4. Diaphragm/Condenser 5. Illuminator (light source) 6. Course Knob 7. Fine Focus knob 8. Reostat (small knob on right hand side) 9. Stage Total Magnification = Ocular Lens (10x) × Objective Lens (4x, 10x, 40x, 100x) Depth-of-Focus : the “Thickness” of the sample that appears. As magnification increases, the actual area of the slide becomes thinner. Field-of-View: the area of the slide you observe. As you increase the magnification, the actual area you are viewing becomes smaller. Working Distance : the distance between the objective and the slide. As you increase magnification, the working distance decreases. Morphology : Shape Arrangement : the way multiple bacterial cells associate and depends on: number of planes of cell division and the extent to which the daughter cells separate. 1. Bacillus (rods with single plane of division) Diplo bacillus (in pairs) Strepto bacillus (in a row/chains) 2. Coccus (circles with infinite planes) Diplo coccus, Strepto coccus, Sarcinae (cubes) Staphylo coccus a coccus with multiple planes of cell division arranged in clusters 3. Spirillum (spirals)
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