Post-Section D ANSWERS Dec 10.docx

Post-Section D ANSWERS Dec 10.docx - Post-Section...

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Post-Section Assignment D Post-Section Assignment D (Released on Wed Nov 29 7pm, due Friday Dec 8 th , 11:59 pm). All answers must be typed, except where noted. Answers must be within the boxes and lines provided, font size must be 11 or greater. Please do not adjust text-box sizes. Below are the answers. This answer key will be updated with a grading rubric once grading of post-section D is complete. You will be notified once you assignment has been graded. Part 1: These past few weeks you have been reading papers in sections. Our TAs have reported the tremendous amount of initiative and engagement you have shown in sections. In the first part of this post-section, we would like you to apply the skills you developed in reading scientific papers. We have selected two figures for you to analyze from the following scientific paper: This paper follows from Hoekstra paper you read, and was published the following year. It further explores genes that affect mouse coat color, but does so on a broader (genome-wide) scale, and also delves into how coat color genes interact with each other. Suggested approach: Read the questions we pose in this post-section first. Then, be strategic by keeping these directed questions in mind and read the paper. You may find the techniques we gave you in pre-section 8 to be helpful. Examine Figure 4B from the above paper, reproduced here for your convenience. 1
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Post-Section Assignment D 2
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Post-Section Assignment D a) What was measured in figure 4B? Underline the ONE BEST choice: A. The amount of Mc1r and Agouti RNA in tissue samples from different Peromyscus subspecies (upper panel), compared with the levels of Mc1r and Agouti protein (shown in the bar graphs in the lower panel). B. The relative amount of Mc1r and Agouti DNA in tissue samples from different Peromyscus subspecies (upper panel). In the bottom panels, to allow comparisons across subspecies, the levels of Mc1r and Agouti DNA are shown in the bar graphs. Data in the bottom panel were transformed so that the tallest bars represent the highest amount of DNA. C. The relative levels of RNA for the Mc1r and Agouti genes in tissue samples from different Peromyscus subspecies (upper panel). The bottom panels represent the levels of actin in the same tissues. Data in the bottom panel were transformed so that the tallest bars represent the highest amount of RNA. D. The levels of RNA for the Actin, Mc1r and Agouti genes in tissue samples from different Peromyscus subspecies (upper panel). The bottom panels represent the same information, but the data were transformed so that the tallest bars represent the highest amount of RNA. E. The levels of RNA for the Actin, Mc1r and Agouti genes in tissue samples from different Peromyscus subspecies (upper panel). In the bottom panel, to allow comparisons across subspecies, the levels of Mc1r and Agouti RNA were plotted relative to a control gene. Data in the bottom panel were transformed so that the tallest bars represent the highest amount of RNA.
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