CarterBrandonLabReport4.pdf - Determining the Better Solvent by Separating Dyes Using Chromatography By Brandon Carter Lab Partner Gabe Neal

CarterBrandonLabReport4.pdf - Determining the Better...

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Determining the Better Solvent by Separating Dyes Using Chromatography By Brandon Carter Lab Partner: Gabe Neal Introduction Purpose Statement: In this lab, you use the method of chromatography to separate various food color dyes. With that, you then determine which of the two solvents between sodium chloride and Isopropyl alcohol, is more efficient when using chromatography. Background: In this lab, you use FD&C dyes that are to be seperated in the paper chromatography. FD&C dyes are dyes approved by the FDA that are used in food to give them color. Chromatography is a method that is often used to separate and thus purify, organic compounds. It functions on the basis that the the compound is soluble in the solvent and will spend time in that solvent but the compound will also spend time in the absorbent. With paper chromatography specifically, it functions the same way as the other methods of chromatography, and uses the paper strip itself as the absorbent. The solvent can be various different solvents. Paper chromatography involves both a stationary phase and a mobile phase. The Stationary phase is essentially just the paper strip that is used to conduct the paper chromatography experiment. The mobile phase in turn, is simply just the solvent. When you place the paper strip into the chromatography chamber, the solvent will move up strip due to capillary action. The molecules that have a similar polarity to the solvent will move up the strip faster. Choosing the right solvent can also be key, because it will determine the level of separation that will be accomplished between the dyes. The intermolecular forces between the solvents and parts of the mixture allow us to seperate the mixtures. This then causes the different mixtures to be separated into parts and then travel separately. Procedures 1. Acquire your three chromatography strips that are 152 mm tall and 19 mm wide. Note: Handle the paper by the edges so the analysis area is not accidentally contaminated by the oils on your skin
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2. Using a ruler and pencil, draw a line across the paper, 20 mm above the bottom of the paper. Then measure 9.5 mm from the edge of the line, and place a dot on the line. This will be the starting point for the sample 3. Now measure 20 mm from the top of the paper and draw another line across the paper. Then fold the paper on that line. This will allow the strip to hang on the lip of the flask. 4. Repeat steps 2 and 3 for the second strip of paper 5. Now obtain the dye mixture 6. Get a toothpick and dip it into the dye mixture. Now touch the tip of the toothpick on the dot on the line of the paper strip and allow the dye to dry on the paper. Repeat this step two more times. Note: This step is necessary to increase the concentration of the sample but don’t allow the size of the spot to increase.
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